C-Fos response to administration of catecholamines into brain by microdialysis

Eric A. Stone, Yi Zhang, Safy M. John, Guoying Bing

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


The present studies investigated the use of microdialysis for the infusion of catecholamines into the brain in studies of immediate early gene (IEG) activation. c-Fos like immunoreactivity was examined histochemically in cortical tissue surrounding a dialysis probe implanted in the medial prefrontal cortex. No c-fos reactivity was observed at 48 h post implantation in control animals not probe-infused or infused 2 h earlier with Ringer's buffer. Marked reactivity was found in animals infused with a solution of norepinephrine (NE). The latter was blocked by coinfusion of the beta adrenoceptor antagonist, timolol. It is concluded that direct infusion of NE by dialysis can induce c-fos expression in cells of the cerebral cortex and that this effect is primarily via β-adrenoceptors. Microdialysis therefore represents a useful technique in studies of IEG responses to brain catecholamines.

Original languageEnglish
Pages (from-to)33-35
Number of pages3
JournalNeuroscience Letters
Issue number1
StatePublished - Nov 25 1991

Bibliographical note

Funding Information:
Supported in part by Grants AFOSR 89-0208, MH45265 and MH08618.


  • Catecholamine
  • Immunohistochemistry
  • Microdialysis
  • NE
  • c-Fos

ASJC Scopus subject areas

  • General Neuroscience


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