TY - JOUR
T1 - Cadmium-induced crystallization of proteins
T2 - II. Crystallization of the Salmonella typhimurium histidine-binding protein in complex with L-histidine, L-arginine, or L-lysine
AU - Trakhanov, Sergei
AU - Kreimer, David I.
AU - Parkin, Sean
AU - Ames, Giovanna Ferro Luzzi
AU - Rupp, Bernhard
PY - 1998/3
Y1 - 1998/3
N2 - To further investigate favorable effects of divalent cations on the formation of protein crystals, three complexes of Salmonella typhimurium histidine-binding protein were crystallized with varying concentrations of cadmium salts. For each of the three histidine-binding protein complexes, cadmium cations were found to promote or improve crystallization. The optimal cadmium concentration is ligand specific and falls within a narrow concentration range. In each case, crystals grown in the presence of cadmium diffract to better than 2.0 Å resolution and belong to the orthorhombic space group P212121. From our results and from the analysis of cadmium sites in well-refined protein structures, we propose that cadmium addition provides a generally useful technique to modify crystal morphology and to improve diffraction quality.
AB - To further investigate favorable effects of divalent cations on the formation of protein crystals, three complexes of Salmonella typhimurium histidine-binding protein were crystallized with varying concentrations of cadmium salts. For each of the three histidine-binding protein complexes, cadmium cations were found to promote or improve crystallization. The optimal cadmium concentration is ligand specific and falls within a narrow concentration range. In each case, crystals grown in the presence of cadmium diffract to better than 2.0 Å resolution and belong to the orthorhombic space group P212121. From our results and from the analysis of cadmium sites in well-refined protein structures, we propose that cadmium addition provides a generally useful technique to modify crystal morphology and to improve diffraction quality.
KW - Cadmium addition
KW - Crystal growth
KW - Crystal morphology
KW - Crystallization
KW - Histidine binding protein
KW - X-ray crystallography
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U2 - 10.1002/pro.5560070308
DO - 10.1002/pro.5560070308
M3 - Article
C2 - 9541391
AN - SCOPUS:0031888667
SN - 0961-8368
VL - 7
SP - 600
EP - 604
JO - Protein Science
JF - Protein Science
IS - 3
ER -