TY - JOUR
T1 - Calcium dependent regulatory protein of cyclic nucleotide metabolism in normal and transformed chicken embryo fibroblasts
AU - Watterson, D. M.
AU - Van Eldik, L. J.
AU - Smith, R. E.
AU - Vanaman, T. C.
PY - 1976
Y1 - 1976
N2 - The concentration of a calcium binding protein modulator of 3':5' cyclic nucleotide phosphodiesterase (3':5' cyclic nucleotide 5' nucleotidohydrolase) activity is increased in chicken embryo fibroblasts upon transformation by Rous sarcoma virus. This modulator protein from fibroblasts, which has roughly the same molecular size, charge, and functional properties as that isolated from chicken brain, comprises approximately 1.32% of the soluble protein in homogenates of fibroblasts infected and transformed by Rous sarcoma virus. In comparison, the modulator comprises approximately 0.30% of the soluble protein in homogenates of normal fibroblasts from confluent cultures and 0.36% of the soluble protein in homogenates of fibroblasts infected with a transformation defective mutant of Rous sarcoma virus. Modulator levels in normal fibroblasts at subconfluent cell densities are 0.42-0.76% of the homogenate soluble protein, i.e., between that found in confluent normal fibroblasts and in fibroblasts transformed by Rous sarcoma virus. These observations suggest that the levels of the modulator protein are elevated under conditions in which chicken embryo fibroblasts are undergoing rapid growth and have decreased adenosine 3':5' cyclic monophosphate levels.
AB - The concentration of a calcium binding protein modulator of 3':5' cyclic nucleotide phosphodiesterase (3':5' cyclic nucleotide 5' nucleotidohydrolase) activity is increased in chicken embryo fibroblasts upon transformation by Rous sarcoma virus. This modulator protein from fibroblasts, which has roughly the same molecular size, charge, and functional properties as that isolated from chicken brain, comprises approximately 1.32% of the soluble protein in homogenates of fibroblasts infected and transformed by Rous sarcoma virus. In comparison, the modulator comprises approximately 0.30% of the soluble protein in homogenates of normal fibroblasts from confluent cultures and 0.36% of the soluble protein in homogenates of fibroblasts infected with a transformation defective mutant of Rous sarcoma virus. Modulator levels in normal fibroblasts at subconfluent cell densities are 0.42-0.76% of the homogenate soluble protein, i.e., between that found in confluent normal fibroblasts and in fibroblasts transformed by Rous sarcoma virus. These observations suggest that the levels of the modulator protein are elevated under conditions in which chicken embryo fibroblasts are undergoing rapid growth and have decreased adenosine 3':5' cyclic monophosphate levels.
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U2 - 10.1073/pnas.73.8.2711
DO - 10.1073/pnas.73.8.2711
M3 - Article
C2 - 183206
AN - SCOPUS:0017062914
SN - 0027-8424
VL - 73
SP - 2711
EP - 2715
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 8
ER -