TY - JOUR
T1 - Calibration of micropipets using the bioluminescent protein aequorin
AU - Grosvenor, Anne L.
AU - Crofcheck, Czarena L.
AU - Anderson, Kimberly W.
AU - Scott, Donna L.
AU - Daunert, Sylvia
PY - 1997/8/1
Y1 - 1997/8/1
N2 - A new method for calibrating micropipets and determining accurate injection volumes using a pressure-based injector has been developed. This method employs the bioluminescent protein aequorin and can be used to determine injection volumes as small as 3 pL. The calibration plots are linear over at least 3 orders of magnitude. In contrast to conventional micropipet calibration methods that employ fluorescent molecules, the present method produces small background signals.
AB - A new method for calibrating micropipets and determining accurate injection volumes using a pressure-based injector has been developed. This method employs the bioluminescent protein aequorin and can be used to determine injection volumes as small as 3 pL. The calibration plots are linear over at least 3 orders of magnitude. In contrast to conventional micropipet calibration methods that employ fluorescent molecules, the present method produces small background signals.
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U2 - 10.1021/ac970123a
DO - 10.1021/ac970123a
M3 - Article
C2 - 9253255
AN - SCOPUS:0031204512
SN - 0003-2700
VL - 69
SP - 3115
EP - 3118
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 15
ER -