Camptothecin effects on DNA synthesis in murine leukemia cells

David Kessel, H. Bruce Bosmann, Kristine Lohr

Research output: Contribution to journalArticlepeer-review

93 Scopus citations

Abstract

The effects of camptothecin on DNA synthesis were investigated using L1210 and L5178Y cells in culture. After addition of the drug, incorporation of labeled thymidine into cell DNA was gradually inhibited; at a 10 μg/ml drug level, 1 h was required to inhibit thymidine incorporation by 95%. Upon removal of drug, this inhibition was slowly reversed over a period of several hours; but the drug-treated cells were incapable of subsequent division. Cells in the S phase of the cell cycle were most sensitive to camptothecin.

Original languageEnglish
Pages (from-to)210-216
Number of pages7
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume269
Issue number2
DOIs
StatePublished - May 10 1972

Bibliographical note

Funding Information:
The technical assistance of Regina Bruns, Kenneth R. Case, and Gerilvn Z. Pike is gratefully acknowledged. This work was supported by grants CA 11198-02 and CA 11242-Ol from the National Institutes of Health, grants TI AM lOO4-O6 (C.I.T.) and GM-I519o from N.I.G.M.S., and grant P529 from the American Cancer Society. H.B.B. is a Reasearch Career Development Awardee of N.I.G.M.S.

ASJC Scopus subject areas

  • Medicine (all)

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