“Candidatus Macondimonas diazotrophica”, a novel gammaproteobacterial genus dominating crude-oil-contaminated coastal sediments

Smruthi Karthikeyan; Luis M. Rodriguez-R; Patrick Heritier-Robbins; Minjae Kim; Will A. Overholt; John C. Gaby; Janet K. Hatt; Jim C. Spain; Ramon Rosselló-Móra; Markus Huettel; Joel E. Kostka; Konstantinos T. Konstantinidis

doi:10.1038/s41396-019-0400-5

“Candidatus Macondimonas diazotrophica”, a novel gammaproteobacterial genus dominating crude-oil-contaminated coastal sediments

Smruthi Karthikeyan, Luis M. Rodriguez-R, Patrick Heritier-Robbins, Minjae Kim, Will A. Overholt, John C. Gaby, Janet K. Hatt, Jim C. Spain, Ramon Rosselló-Móra, Markus Huettel, Joel E. Kostka, Konstantinos T. Konstantinidis

Civil Engineering

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Modeling crude-oil biodegradation in sediments remains a challenge due in part to the lack of appropriate model organisms. Here we report the metagenome-guided isolation of a novel organism that represents a phylogenetically narrow (>97% 16S rRNA gene identity) group of previously uncharacterized, crude-oil degraders. Analysis of available sequence data showed that these organisms are highly abundant in oiled sediments of coastal marine ecosystems across the world, often comprising ~30% of the total community, and virtually absent in pristine sediments or seawater. The isolate genome encodes functional nitrogen fixation and hydrocarbon degradation genes together with putative genes for biosurfactant production that apparently facilitate growth in the typically nitrogen-limited, oiled environment. Comparisons to available genomes revealed that this isolate represents a novel genus within the Gammaproteobacteria, for which we propose the provisional name “Candidatus Macondimonas diazotrophica” gen. nov., sp. nov. “Ca. M. diazotrophica” appears to play a key ecological role in the response to oil spills around the globe and could be a promising model organism for studying ecophysiological responses to oil spills.

Original language	English
Pages (from-to)	2129-2134
Number of pages	6
Journal	ISME Journal
Volume	13
Issue number	8
DOIs	https://doi.org/10.1038/s41396-019-0400-5
State	Published - Aug 1 2019

Bibliographical note

Funding Information:
Acknowledgements The authors would like to thank Dr. Aharon Oren for his valuable input on the naming of the new type species. This research was made possible by a grant from The Gulf of Mexico Research Initiative (Grant No 321611-00; RFP V). Data are publicly available through the Gulf of Mexico Research Initiative Information & Data Cooperative (GRIIDC) at https://data.gulfresearchinitiative.org (UDI: R5.x278.000:0014, UDI: R5.x278.000:0002). RR-M acknowledges the financial support for his sabbatical stay at Georgia Tech from the Spanish Ministry of Sciences, Innovation and Universities (Grant No. PRX18/00048).

Publisher Copyright:
© 2019, International Society for Microbial Ecology.

ASJC Scopus subject areas

Microbiology
Ecology, Evolution, Behavior and Systematics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/s41396-019-0400-5

Cite this

Karthikeyan, S., Rodriguez-R, L. M., Heritier-Robbins, P., Kim, M., Overholt, W. A., Gaby, J. C., Hatt, J. K., Spain, J. C., Rosselló-Móra, R., Huettel, M., Kostka, J. E., & Konstantinidis, K. T. (2019). “Candidatus Macondimonas diazotrophica”, a novel gammaproteobacterial genus dominating crude-oil-contaminated coastal sediments. ISME Journal, 13(8), 2129-2134. https://doi.org/10.1038/s41396-019-0400-5

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title = "“Candidatus Macondimonas diazotrophica”, a novel gammaproteobacterial genus dominating crude-oil-contaminated coastal sediments",

abstract = "Modeling crude-oil biodegradation in sediments remains a challenge due in part to the lack of appropriate model organisms. Here we report the metagenome-guided isolation of a novel organism that represents a phylogenetically narrow (>97% 16S rRNA gene identity) group of previously uncharacterized, crude-oil degraders. Analysis of available sequence data showed that these organisms are highly abundant in oiled sediments of coastal marine ecosystems across the world, often comprising ~30% of the total community, and virtually absent in pristine sediments or seawater. The isolate genome encodes functional nitrogen fixation and hydrocarbon degradation genes together with putative genes for biosurfactant production that apparently facilitate growth in the typically nitrogen-limited, oiled environment. Comparisons to available genomes revealed that this isolate represents a novel genus within the Gammaproteobacteria, for which we propose the provisional name “Candidatus Macondimonas diazotrophica” gen. nov., sp. nov. “Ca. M. diazotrophica” appears to play a key ecological role in the response to oil spills around the globe and could be a promising model organism for studying ecophysiological responses to oil spills.",

author = "Smruthi Karthikeyan and Rodriguez-R, {Luis M.} and Patrick Heritier-Robbins and Minjae Kim and Overholt, {Will A.} and Gaby, {John C.} and Hatt, {Janet K.} and Spain, {Jim C.} and Ramon Rossell{\'o}-M{\'o}ra and Markus Huettel and Kostka, {Joel E.} and Konstantinidis, {Konstantinos T.}",

note = "Funding Information: Acknowledgements The authors would like to thank Dr. Aharon Oren for his valuable input on the naming of the new type species. This research was made possible by a grant from The Gulf of Mexico Research Initiative (Grant No 321611-00; RFP V). Data are publicly available through the Gulf of Mexico Research Initiative Information & Data Cooperative (GRIIDC) at https://data.gulfresearchinitiative.org (UDI: R5.x278.000:0014, UDI: R5.x278.000:0002). RR-M acknowledges the financial support for his sabbatical stay at Georgia Tech from the Spanish Ministry of Sciences, Innovation and Universities (Grant No. PRX18/00048). Publisher Copyright: {\textcopyright} 2019, International Society for Microbial Ecology.",

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Karthikeyan, S, Rodriguez-R, LM, Heritier-Robbins, P, Kim, M, Overholt, WA, Gaby, JC, Hatt, JK, Spain, JC, Rosselló-Móra, R, Huettel, M, Kostka, JE & Konstantinidis, KT 2019, '“Candidatus Macondimonas diazotrophica”, a novel gammaproteobacterial genus dominating crude-oil-contaminated coastal sediments', ISME Journal, vol. 13, no. 8, pp. 2129-2134. https://doi.org/10.1038/s41396-019-0400-5

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AU - Konstantinidis, Konstantinos T.

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N2 - Modeling crude-oil biodegradation in sediments remains a challenge due in part to the lack of appropriate model organisms. Here we report the metagenome-guided isolation of a novel organism that represents a phylogenetically narrow (>97% 16S rRNA gene identity) group of previously uncharacterized, crude-oil degraders. Analysis of available sequence data showed that these organisms are highly abundant in oiled sediments of coastal marine ecosystems across the world, often comprising ~30% of the total community, and virtually absent in pristine sediments or seawater. The isolate genome encodes functional nitrogen fixation and hydrocarbon degradation genes together with putative genes for biosurfactant production that apparently facilitate growth in the typically nitrogen-limited, oiled environment. Comparisons to available genomes revealed that this isolate represents a novel genus within the Gammaproteobacteria, for which we propose the provisional name “Candidatus Macondimonas diazotrophica” gen. nov., sp. nov. “Ca. M. diazotrophica” appears to play a key ecological role in the response to oil spills around the globe and could be a promising model organism for studying ecophysiological responses to oil spills.

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“Candidatus Macondimonas diazotrophica”, a novel gammaproteobacterial genus dominating crude-oil-contaminated coastal sediments

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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