Cap n collar transcription factor regulates multiple genes coding for proteins involved in insecticide detoxification in the red flour beetle, Tribolium castaneum

Megha Kalsi; Subba Reddy Palli

doi:10.1016/j.ibmb.2017.09.009

Cap n collar transcription factor regulates multiple genes coding for proteins involved in insecticide detoxification in the red flour beetle, Tribolium castaneum

Megha Kalsi, Subba Reddy Palli

Research output: Contribution to journal › Article › peer-review

79 Scopus citations

Abstract

In invertebrates, a heterodimer of xenobiotic transcription factors, cap n collar C isoform (CncC) and muscle aponeurosis fibromatosis (Maf) mediate cellular defense. In insects, these proteins regulate expression of genes involved in insecticide detoxification. In the current study, we performed sequencing of cDNA copied from RNA isolated from Tribolium castaneum pyrethroid resistant strain (QTC279) beetles injected with CncC or green fluorescence protein (GFP, control) dsRNA. Differential expression analysis of sequences identified 662 genes that showed a decrease and 91 genes that showed an increase in expression (p value ≤ 0.01 and log2 fold change of ≥ 1.5) in CncC knockdown insects when compared to their expression in control insects. We selected a subset of 27 downregulated genes and verified their differential expression using qRT-PCR. This subset of 27 genes included 21 genes with a predicted function in xenobiotic detoxification. RNAi and insecticide bioassays were employed to study the function of six of these genes coding for CYP4G7, CYP4G14, GST-1 and four ABC transporters, ABCA-UB, ABCA-A1 and ABCA-A1L and ABCA-9B involved in all three phases of insecticide detoxification. These data suggest that CncC regulates genes coding for proteins involved in detoxification of insecticides.

Original language	English
Pages (from-to)	43-52
Number of pages	10
Journal	Insect Biochemistry and Molecular Biology
Volume	90
DOIs	https://doi.org/10.1016/j.ibmb.2017.09.009
State	Published - Nov 2017

Bibliographical note

Publisher Copyright:
© 2017 Elsevier Ltd

Funding

This work was supported by USDA-NRI-CSREES ( 2011-04636 ). This is publication number 17-08-094 from the Kentucky Agricultural Experimental Station and published with the approval of the director. This work is supported by the National Institute of Food and Agriculture, USDA, HATCH under 2351177000 . We would like to thank Dr. Arthur Hunt in plant and soil science department, University of Kentucky and Smitha George for help in preparing the RNA-seq libraries.

Funders	Funder number
NRI/CSREES/USDA	2011-04636
U.S. Department of Agriculture	2351177000
U.S. Department of Agriculture
US Department of Agriculture National Institute of Food and Agriculture, Agriculture and Food Research Initiative

Keywords

ABC transporter
GST
P450
RNA-seq
Red flour beetle

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Insect Science

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10.1016/j.ibmb.2017.09.009

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title = "Cap n collar transcription factor regulates multiple genes coding for proteins involved in insecticide detoxification in the red flour beetle, Tribolium castaneum",

abstract = "In invertebrates, a heterodimer of xenobiotic transcription factors, cap n collar C isoform (CncC) and muscle aponeurosis fibromatosis (Maf) mediate cellular defense. In insects, these proteins regulate expression of genes involved in insecticide detoxification. In the current study, we performed sequencing of cDNA copied from RNA isolated from Tribolium castaneum pyrethroid resistant strain (QTC279) beetles injected with CncC or green fluorescence protein (GFP, control) dsRNA. Differential expression analysis of sequences identified 662 genes that showed a decrease and 91 genes that showed an increase in expression (p value ≤ 0.01 and log2 fold change of ≥ 1.5) in CncC knockdown insects when compared to their expression in control insects. We selected a subset of 27 downregulated genes and verified their differential expression using qRT-PCR. This subset of 27 genes included 21 genes with a predicted function in xenobiotic detoxification. RNAi and insecticide bioassays were employed to study the function of six of these genes coding for CYP4G7, CYP4G14, GST-1 and four ABC transporters, ABCA-UB, ABCA-A1 and ABCA-A1L and ABCA-9B involved in all three phases of insecticide detoxification. These data suggest that CncC regulates genes coding for proteins involved in detoxification of insecticides.",

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doi = "10.1016/j.ibmb.2017.09.009",

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N2 - In invertebrates, a heterodimer of xenobiotic transcription factors, cap n collar C isoform (CncC) and muscle aponeurosis fibromatosis (Maf) mediate cellular defense. In insects, these proteins regulate expression of genes involved in insecticide detoxification. In the current study, we performed sequencing of cDNA copied from RNA isolated from Tribolium castaneum pyrethroid resistant strain (QTC279) beetles injected with CncC or green fluorescence protein (GFP, control) dsRNA. Differential expression analysis of sequences identified 662 genes that showed a decrease and 91 genes that showed an increase in expression (p value ≤ 0.01 and log2 fold change of ≥ 1.5) in CncC knockdown insects when compared to their expression in control insects. We selected a subset of 27 downregulated genes and verified their differential expression using qRT-PCR. This subset of 27 genes included 21 genes with a predicted function in xenobiotic detoxification. RNAi and insecticide bioassays were employed to study the function of six of these genes coding for CYP4G7, CYP4G14, GST-1 and four ABC transporters, ABCA-UB, ABCA-A1 and ABCA-A1L and ABCA-9B involved in all three phases of insecticide detoxification. These data suggest that CncC regulates genes coding for proteins involved in detoxification of insecticides.

AB - In invertebrates, a heterodimer of xenobiotic transcription factors, cap n collar C isoform (CncC) and muscle aponeurosis fibromatosis (Maf) mediate cellular defense. In insects, these proteins regulate expression of genes involved in insecticide detoxification. In the current study, we performed sequencing of cDNA copied from RNA isolated from Tribolium castaneum pyrethroid resistant strain (QTC279) beetles injected with CncC or green fluorescence protein (GFP, control) dsRNA. Differential expression analysis of sequences identified 662 genes that showed a decrease and 91 genes that showed an increase in expression (p value ≤ 0.01 and log2 fold change of ≥ 1.5) in CncC knockdown insects when compared to their expression in control insects. We selected a subset of 27 downregulated genes and verified their differential expression using qRT-PCR. This subset of 27 genes included 21 genes with a predicted function in xenobiotic detoxification. RNAi and insecticide bioassays were employed to study the function of six of these genes coding for CYP4G7, CYP4G14, GST-1 and four ABC transporters, ABCA-UB, ABCA-A1 and ABCA-A1L and ABCA-9B involved in all three phases of insecticide detoxification. These data suggest that CncC regulates genes coding for proteins involved in detoxification of insecticides.

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Cap n collar transcription factor regulates multiple genes coding for proteins involved in insecticide detoxification in the red flour beetle, Tribolium castaneum

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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