Cardioprotective effect of total paeony glycosides against isoprenaline-induced myocardial ischemia in rats

Jiangang Long, Meili Gao, Yu Kong, Xian Shen, Xiaoyang Du, Young Ok Son, Xianglin Shi, Jiankang Liu, Xiaoyan Mo

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Paeoniae radix is a traditional Chinese medicinal herb for treating some diseases; important components are total paeony glycosides (TPGs), an approved drug by the State Food and Drug Administration (SFDA) for the therapy of rheumatoid arthritis (RA). We firstly reported myocardial benefits of TPGs previously, and the present study is to further investigate the underlying mechanisms for preventing oxidative damage in cardiomyopathy. We measured the capacity of TPGs to scavenge free radicals in vitro. Then 60 SD rats were randomly divided into five groups: (1) a normal control group, (2) an isoprenaline (ISO)-induced myocardial ischemic model group, (3) a TPG treatment group (TPGs 269.4 mg/kg delivered by intragastric administration for 3 days before ISO administration and TPGs 449 mg/kg delivered for 3 days after ISO administration), (4) a TPG therapy group (TPGs 449 mg/kg delivered for 3 days after ISO administration), and (5) a positive control group (propranolol 15 mg/kg for 3 days after ISO administration). The ISO-induced myocardial ischemic model was established by subcutaneous injection of 1 mg/kg/8 h ISO (2 times). The activities of myocardial enzymes, including glutamic oxaloacetic transaminase (GOT), creatine kinase (CK), lactate dehydrogenase (LDH), antioxidant enzyme superoxide dismutase (SOD) as well as the content of lipid peroxidation product malondialdehyde (MDA) were detected. We found that TPGs potently eliminated hydroxyl radicals and superoxide in vitro using ESR assays. Compared with model rats, TPG treatment, TPG therapy and the positive control treatment exhibited significantly reduced activities of GOT, LDH, and CK (p < 0.01), increased activity of SOD (p < 0.01) and lower levels of MDA (p < 0.05). More interestingly, the protective effect of TPG treatment was even better than that of propranolol. These results suggest that TPGs significantly ameliorate ISO-induced myocardial ischemia and their action might be through reducing oxidative stress in ischemic myocardium.

Original languageEnglish
Pages (from-to)672-676
Number of pages5
JournalPhytomedicine
Volume19
Issue number8-9
DOIs
StatePublished - Jun 15 2012

Bibliographical note

Funding Information:
Authors thank Dr. Edward Sharman, University of California for his critical reading and language editing. This work was partially supported by Foundation of Xi’an Jiaotong University, Program for New Century Excellent Talents in University, and the National Natural Science Foundation of China (Grant No. 31070740 ).

Keywords

  • Free radicals
  • Myocardial enzymes
  • Myocardial ischemia
  • Paeonia rubra
  • Total paeony glycosides

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine

Fingerprint

Dive into the research topics of 'Cardioprotective effect of total paeony glycosides against isoprenaline-induced myocardial ischemia in rats'. Together they form a unique fingerprint.

Cite this