Caspase activation in equine influenza virus induced apoptotic cell death

Chengbin Lin, Robert E. Holland, Jennifer C. Donofrio, Morgan H. McCoy, Lynn R. Tudor, Thomas M. Chambers

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Equine influenza virus (EIV) is the leading cause of acute respiratory infection in horses worldwide. In recent years, the precise mechanism by which influenza infection kills host cells is being re-evaluated. In this report, we examined whether caspases, a group of intracellular proteases, are activated following EIV infection and contribute to EIV-mediated cell death. Western blotting analysis indicated that a nuclear target of caspase-3, poly(ADP-ribose) polymerase (PARP) was proteolytically cleaved in EIV-infected MDCK cells, but not in mock-infected cells. In comparison with caspase-3 specific inhibitor Ac-DEVD-CHO, a general caspase inhibitor Boc-D-FMK provided much stronger inhibition of EIV-induced cytopathic effect and apoptosis. Our results suggest that EIV may activate more than one caspase. Caspase activation and cleavage of its cellular targets may play a critical role in EIV-mediated cytotoxicity.

Original languageEnglish
Pages (from-to)357-365
Number of pages9
JournalVeterinary Microbiology
Volume84
Issue number4
DOIs
StatePublished - Feb 4 2002

Bibliographical note

Funding Information:
We wish to acknowledge Dr. Stephen Zimmer (Department of Microbiology and Immunology, University of Kentucky School of Medicine) for his helpful comments on this manuscript. We thank Dr. Judith Appleton (James A. Baker Institute for Animal Health, Cornell University) for the generous gift of F90 5 H1.25 antibody. We also thank Dr. Shu-Chen Cheng and Ms. Linda Obenauer-Kutner (Schering-Plough Research Institute) for help with the glossy prints. This work was supported in part by USDA/CRS project no. KY 014006. The investigation reported in this paper is in connection with a project of the Kentucky Agricultural Experiment Station (no. 99-14-107) and is published with approval of the director.

Keywords

  • Ac-DEVD-CHO
  • Boc-D-FMK
  • Caspases
  • Equine influenza virus (EIV)
  • Madin-Darby canine kidney (MDCK) cells
  • Poly(ADP-ribose) polymerase (PARP)

ASJC Scopus subject areas

  • Microbiology
  • Veterinary (all)

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