TY - JOUR
T1 - Ca2+/calmodulin-dependent protein kinases from the neuronal nuclear matrix and post-synaptic density are structurally related
AU - Sahyoun, N.
AU - LeVine, H.
AU - Cuatrecasas, P.
PY - 1984
Y1 - 1984
N2 - A major Ca2+/calmodulin-dependent protein kinase has been isolated in association with the neuronal nuclear matrix. Nuclear matrix preparations contain highly phosphorylated polypeptides with M(r) values of 50,000 and 60,000. These polypeptides were further characterized by peptide and phospho peptide mapping, two-dimensional isoelectrofocusing/NaDodSO4/PAGE, and 125I-labeled calmodulin binding. The results indicate that the M(r) 50,000 and 60,000 polypeptides of the nuclear matrix closely resemble the α and β subunits, respectively, of the Ca2+/calmodulin-dependent protein kinase of the post-synaptic density. These findings indicate that similar protein kinases mediate the neuronal effects of Ca2+ at the cytosolic, synaptosomal, and nuclear levels.
AB - A major Ca2+/calmodulin-dependent protein kinase has been isolated in association with the neuronal nuclear matrix. Nuclear matrix preparations contain highly phosphorylated polypeptides with M(r) values of 50,000 and 60,000. These polypeptides were further characterized by peptide and phospho peptide mapping, two-dimensional isoelectrofocusing/NaDodSO4/PAGE, and 125I-labeled calmodulin binding. The results indicate that the M(r) 50,000 and 60,000 polypeptides of the nuclear matrix closely resemble the α and β subunits, respectively, of the Ca2+/calmodulin-dependent protein kinase of the post-synaptic density. These findings indicate that similar protein kinases mediate the neuronal effects of Ca2+ at the cytosolic, synaptosomal, and nuclear levels.
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U2 - 10.1073/pnas.81.14.4311
DO - 10.1073/pnas.81.14.4311
M3 - Article
C2 - 6589595
AN - SCOPUS:0021174889
SN - 0027-8424
VL - 81
SP - 4311
EP - 4315
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 14 I
ER -