CCT chaperonin complex is required for the biogenesis of functional Plk1

Xiaoqi Liu, Chin Yo Lin, Ming Lei, Shi Yan, Tianhua Zhou, Raymond L. Erikson

Research output: Contribution to journalArticlepeer-review

62 Scopus citations


Experiments from several different organisms have demonstrated that polo-like kinases are involved in many aspects of mitosis and cytokinesis. Here, we provide evidence to show that Plk1 associates with chaperonin-containing TCP1 complex (CCT) both in vitro and in vivo. Silencing of CCT by use of RNA interference (RNAi) in mammalian cells inhibits cell proliferation, decreases cell viability, causes cell cycle arrest with 4N DNA content, and leads to apoptosis. Depletion of CCT in well-synchronized HeLa cells causes cell cycle arrest at G2, as demonstrated by a low miitotic index and Cdc2 activity. Complete depletion of Plk1 in well-synchronized cells also leads to G2 block, suggesting that misfolded Plk1 might be responsible for the failure of CCT-depleted cells to enter mitosis. Moreover, partial depletion of CCT or Plk1 leads to mitotic arrest. Finally, the CCT-depleted cells reenter the cell cycle upon reintroduction of the purified constitutively active form of Plk1, indicating that Plk1 might be a CCT substrate.

Original languageEnglish
Pages (from-to)4993-5010
Number of pages18
JournalMolecular and Cellular Biology
Issue number12
StatePublished - Jun 2005

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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