Background: Tetraspanins CD151, a transmembrane 4 superfamily protein, has been identified participating in the initiation of a variety of cancers. However, the precise function of CD151 in non-small cell lung cancer (NSCLC) remains unclear. Here, we addressed the pro-tumoral role of CD151 in NSCLC by targeting EGFR/ErbB2 which favors tumor proliferation, migration and invasion. Methods: First, the mRNA expression levels of CD151 in NSCLC tissues and cell lines were measured by RT-PCR. Meanwhile, CD151 and its associated proteins were analyzed by western blotting. The expression levels of CD151 in NSCLC samples and its paired adjacent lung tissues were then verified by Immunohistochemistry. The protein interactions are evaluated by co-immunoprecipitation. Flow cytometry was applied to cell cycle analysis. CCK-8, EdU Incorporation, and clonogenic assays were used to analyze cell viability. Wound healing, transwell migration, and matrigel invasion assays were utilized to assess the motility of tumor cells. To investigate the role of CD151 in vivo, lung carcinoma xenograft mouse model was applied. Results: High CD151 expression was identified in NSCLC tissues and cell lines, and its high expression was significantly associated with poor prognosis of NSCLC patients. Further, knockdown of CD151 in vitro inhibited tumor proliferation, migration, and invasion. Besides, inoculation of nude mice with CD151-overexpressing tumor cells exhibited substantial tumor proliferation compared to that in control mice which inoculated with vector-transfected tumor cells. Noteworthy, we found that overexpression of CD151 conferred cell migration and invasion by interacting with integrins. We next sought to demonstrate that CD151 regulated downstream signaling pathways via activation of EGFR/ErbB2 in NSCLC cells. Therefore, we infer that CD151 probably affects the sensitivity of NSCLC in response to anti-cancer drugs. Conclusions: Based on these results, we demonstrated a new mechanism of CD151-mediated tumor progression by targeting EGFR/ErbB2 signaling pathway, by which CD151 promotes NSCLC proliferation, migration, and invasion, which may considered as a potential target of NSCLC treatment.
|Journal||Journal of Experimental and Clinical Cancer Research|
|State||Published - Dec 2021|
Bibliographical noteFunding Information:
We thank all patients who participated in this study for their cooperation, and Dr. Dongsheng Pei for analysis of immunohistochemical results at Department of Pathology, Xuzhou Medical University, Xuzhou, China.
This work was supported by grants from National Natural Science Foundation of China (81702870 and 81802885 and 82073213), Jiangsu Provincial Medical Youth Talent (No. QNRC2016746), the Suzhou Gusu Medical Youth Talent (GSWS2020016), Science and Technology Plan Project of Suzhou (No. SYS201749), Suzhou Key Laboratory for Respiratory Medicine (No. SZS201617), Clinical Medical Center of Suzhou (No.Szzx201502), Jiangsu Provincial Key Medical Discipline (No. ZDXKB2016007), Medical and health science and technology project in Zhejiang Province (2019ZD024), Natural science research program of Huai’an city (No. HAB201813).
© 2021, The Author(s).
ASJC Scopus subject areas
- Cancer Research