Cell death persists in rapid extrusion of lysis-resistant coated cardiac myoblasts

Calvin F. Cahall, Aman Preet Kaur, Kara A. Davis, Jonathan T. Pham, Hainsworth Y. Shin, Brad J. Berron

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


As the demand for organ transplants continues to grow faster than the supply of available donor organs, a new source of functional organs is needed. High resolution high throughput 3D bioprinting is one approach towards generating functional organs for transplantation. For high throughput printing, the need for increased print resolutions (by decreasing printing nozzle diameter) has a consequence: it increases the forces that cause cell damage during the printing process. Here, a novel cell encapsulation method provides mechanical protection from complete lysis of individual living cells during extrusion-based bioprinting. Cells coated in polymers possessing the mechanical properties finely-tuned to maintain size and shape following extrusion, and these encapsulated cells are protected from mechanical lysis. However, the shear forces imposed on the cells during extrusion still cause sufficient damage to compromise the cell membrane integrity and adversely impact normal cellular function. Cellular damage occurred during the extrusion process independent of the rapid depressurization.

Original languageEnglish
Article numbere00072
StatePublished - Jun 2020

Bibliographical note

Publisher Copyright:
© 2020 Elsevier B.V.


  • Encapsulation
  • Extrusion
  • Hydrogel
  • Photopolymerization
  • Shear

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering
  • Computer Science Applications


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