Cellular and morphological alterations in the vastus lateralis muscle as the result of ACL injury and reconstruction

Brian Noehren, Anders Andersen, Peter Hardy, Darren L. Johnson, Mary Lloyd Ireland, Katherine L. Thompson, Bruce Damon

Research output: Contribution to journalArticlepeer-review

78 Scopus citations


Background: Individuals who have had an anterior cruciate ligament (ACL) tear and reconstruction continue to experience substantial knee extensor strength loss despite months of physical therapy. Identification of the alterations in muscle morphology and cellular composition are needed to understand potential mechanisms of muscle strength loss, initially as the result of the injury and subsequently from surgery and rehabilitation. Methods: We performed diffusion tensor imaging-magnetic resonance imaging and analyzed muscle biopsies from the vastus lateralis of both the affected and unaffected limbs before surgery and again from the reconstructed limb following the completion of rehabilitation. Immunohistochemistry was done to determine fiber type and size, Pax-7-positive (satellite) cells, and extracellular matrix (via wheat germ agglutinin straining). Using the diffusion tensor imaging data, the fiber tract length, pennation angle, and muscle volume were determined, yielding the physiological cross-sectional area (PCSA). Paired t tests were used to compare the effects of the injury between injured and uninjured limbs and the effects of surgery and rehabilitation within the injured limb. Results: We found significant reductions before surgery in type-IIA muscle cross-sectional area (CSA; p = 0.03), extracellular matrix (p < 0.01), satellite cells per fiber (p < 0.01), pennation angle (p = 0.03),muscle volume (p = 0.02), and PCSA (p = 0.03) in the injured limb compared with the uninjured limb. Following surgery, these alterations in the injured limb persisted and the frequency of the IIA fiber type decreased significantly (p < 0.01) and that of the IIA/X hybrid fiber type increased significantly (p < 0.01). Conclusions: Significant and prolonged differences in muscle quality and morphology occurred after ACL injury and persisted despite reconstruction and extensive physical therapy. Clinical Relevance: These results suggest the need to develop more effective early interventions following an ACL tear to prevent deleterious alterations within the quadriceps.

Original languageEnglish
Pages (from-to)1541-1547
Number of pages7
JournalJournal of Bone and Joint Surgery - American Volume
Issue number18
StatePublished - 2016

Bibliographical note

Publisher Copyright:
copyright © 2016 by the journal of Bone and joint surgery, incorporated.

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine


Dive into the research topics of 'Cellular and morphological alterations in the vastus lateralis muscle as the result of ACL injury and reconstruction'. Together they form a unique fingerprint.

Cite this