Ceramic-based multisite microelectrode array for rapid choline measures in brain tissue

Jason J. Burmeister; Michael Palmer; Greg A. Gerhardt

doi:10.1016/S0003-2670(03)00067-9

Ceramic-based multisite microelectrode array for rapid choline measures in brain tissue

Jason J. Burmeister, Michael Palmer, Greg A. Gerhardt

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

A ceramic-based multisite microelectrode array to measure choline in vivo in brain tissues is described. The microelectrodes were linear to 200μM choline (R²=0.999±0.001) with a detection limit of approximately 0.4μM (S/N of 3) in both single microelectrode and self-referencing amperometric recording modes. The 90% rise time of the sensor was 1.4s, allowing for rapid measures of choline. Good selectivity (>300:1) was observed over interferents such as ascorbic acid, uric acid, and DOPAC in the single microelectrode mode. However, a self-referencing recording mode was needed to remove potassium-evoked dopamine signals in rat striatum. In vivo measurements of choline in the rat brain are presented.

Original language	English
Pages (from-to)	65-74
Number of pages	10
Journal	Analytica Chimica Acta
Volume	481
Issue number	1
DOIs	https://doi.org/10.1016/S0003-2670(03)00067-9
State	Published - Mar 28 2003

Bibliographical note

Funding Information:
This work is supported by grants NSF (DBI 9987807), (AA11465), NIMH (MH-01245), and NIDA (DA14944).

Funding

This work is supported by grants NSF (DBI 9987807), (AA11465), NIMH (MH-01245), and NIDA (DA14944).

Funders	Funder number
National Science Foundation (NSF)	DBI 9987807, AA11465
National Institute of Mental Health	MH-01245
National Institute on Drug Abuse	DA14944

Keywords

Biosensor
CNS
Choline
Neurotransmitters
Voltammetry

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Spectroscopy
Environmental Chemistry

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10.1016/S0003-2670(03)00067-9

Cite this

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title = "Ceramic-based multisite microelectrode array for rapid choline measures in brain tissue",

abstract = "A ceramic-based multisite microelectrode array to measure choline in vivo in brain tissues is described. The microelectrodes were linear to 200μM choline (R2=0.999±0.001) with a detection limit of approximately 0.4μM (S/N of 3) in both single microelectrode and self-referencing amperometric recording modes. The 90% rise time of the sensor was 1.4s, allowing for rapid measures of choline. Good selectivity (>300:1) was observed over interferents such as ascorbic acid, uric acid, and DOPAC in the single microelectrode mode. However, a self-referencing recording mode was needed to remove potassium-evoked dopamine signals in rat striatum. In vivo measurements of choline in the rat brain are presented.",

keywords = "Biosensor, CNS, Choline, Neurotransmitters, Voltammetry",

author = "Burmeister, {Jason J.} and Michael Palmer and Gerhardt, {Greg A.}",

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AU - Burmeister, Jason J.

AU - Palmer, Michael

AU - Gerhardt, Greg A.

N1 - Funding Information: This work is supported by grants NSF (DBI 9987807), (AA11465), NIMH (MH-01245), and NIDA (DA14944).

PY - 2003/3/28

Y1 - 2003/3/28

N2 - A ceramic-based multisite microelectrode array to measure choline in vivo in brain tissues is described. The microelectrodes were linear to 200μM choline (R2=0.999±0.001) with a detection limit of approximately 0.4μM (S/N of 3) in both single microelectrode and self-referencing amperometric recording modes. The 90% rise time of the sensor was 1.4s, allowing for rapid measures of choline. Good selectivity (>300:1) was observed over interferents such as ascorbic acid, uric acid, and DOPAC in the single microelectrode mode. However, a self-referencing recording mode was needed to remove potassium-evoked dopamine signals in rat striatum. In vivo measurements of choline in the rat brain are presented.

AB - A ceramic-based multisite microelectrode array to measure choline in vivo in brain tissues is described. The microelectrodes were linear to 200μM choline (R2=0.999±0.001) with a detection limit of approximately 0.4μM (S/N of 3) in both single microelectrode and self-referencing amperometric recording modes. The 90% rise time of the sensor was 1.4s, allowing for rapid measures of choline. Good selectivity (>300:1) was observed over interferents such as ascorbic acid, uric acid, and DOPAC in the single microelectrode mode. However, a self-referencing recording mode was needed to remove potassium-evoked dopamine signals in rat striatum. In vivo measurements of choline in the rat brain are presented.

KW - Biosensor

KW - CNS

KW - Choline

KW - Neurotransmitters

KW - Voltammetry

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Ceramic-based multisite microelectrode array for rapid choline measures in brain tissue

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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