TY - JOUR
T1 - Ceramide-1-phosphate regulates migration of multipotent stromal cells and endothelial progenitor cells - Implications for tissue regeneration
AU - Kim, Chihwa
AU - Schneider, Gabriela
AU - Abdel-Latif, Ahmed
AU - Mierzejewska, Kasia
AU - Sunkara, Manjula
AU - Borkowska, Sylwia
AU - Ratajczak, Janina
AU - Morris, Andrew J.
AU - Kucia, Magda
AU - Ratajczak, Mariusz Z.
PY - 2013/3
Y1 - 2013/3
N2 - Ceramide-1-phosphate (C1P) is a bioactive lipid that, in contrast to ceramide, is an antiapoptotic molecule released from cells that are damaged and "leaky." As reported recently, C1P promotes migration of hematopoietic cells. In this article, we tested the hypothesis that C1P released upon tissue damage may play an underappreciated role in chemoattraction of various types of stem cells and endothelial cells involved in tissue/organ regeneration. We show for the first time that C1P is upregulated in damaged tissues and chemoattracts bone marrow (BM)-derived multipotent stromal cells, endothelial progenitor cells, and very small embryonic- like stem cells. Furthermore, compared to other bioactive lipids, C1P more potently chemoattracted human umbilical vein endothelial cells and stimulated tube formation by these cells. C1P also promoted in vivo vascularization of Matrigel implants and stimulated secretion of stromal cell-derived factor-1 from BM-derived fibroblasts. Thus, our data demonstrate, for the first time, that C1P is a potent bioactive lipid released from damaged cells that potentially plays an important and novel role in recruitment of stem/progenitor cells to damaged organs and may promote their vascularization.
AB - Ceramide-1-phosphate (C1P) is a bioactive lipid that, in contrast to ceramide, is an antiapoptotic molecule released from cells that are damaged and "leaky." As reported recently, C1P promotes migration of hematopoietic cells. In this article, we tested the hypothesis that C1P released upon tissue damage may play an underappreciated role in chemoattraction of various types of stem cells and endothelial cells involved in tissue/organ regeneration. We show for the first time that C1P is upregulated in damaged tissues and chemoattracts bone marrow (BM)-derived multipotent stromal cells, endothelial progenitor cells, and very small embryonic- like stem cells. Furthermore, compared to other bioactive lipids, C1P more potently chemoattracted human umbilical vein endothelial cells and stimulated tube formation by these cells. C1P also promoted in vivo vascularization of Matrigel implants and stimulated secretion of stromal cell-derived factor-1 from BM-derived fibroblasts. Thus, our data demonstrate, for the first time, that C1P is a potent bioactive lipid released from damaged cells that potentially plays an important and novel role in recruitment of stem/progenitor cells to damaged organs and may promote their vascularization.
KW - Angiogenesis
KW - Ceramide-1-phosphate
KW - Chemotaxis
KW - Human umbilical vein endothelial cell
KW - Multipotent stromal cell
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UR - http://www.scopus.com/inward/citedby.url?scp=84874855328&partnerID=8YFLogxK
U2 - 10.1002/stem.1291
DO - 10.1002/stem.1291
M3 - Article
C2 - 23193025
AN - SCOPUS:84874855328
SN - 1066-5099
VL - 31
SP - 500
EP - 510
JO - Stem Cells
JF - Stem Cells
IS - 3
ER -
