Bioactive sphingolipids are important regulators for stem cell survival and differentiation. Most recently, we have coined the term “morphogenetic lipids” for sphingolipids that regulate stem cells during embryonic and postnatal development. The sphingolipid ceramide and its derivative, sphingosine-1-phosphate (S1P), can act synergistically as well as antagonistically on embryonic stem (ES) cell differentiation. We show here simple as well as state-of-the-art methods to analyze sphingolipids in differentiating ES cells and discuss new protocols to use ceramide and S1P analogs for the guided differentiation of mouse ES cells toward neuronal and glial lineage.
|Title of host publication||Methods in Molecular Biology|
|Number of pages||19|
|State||Published - 2018|
|Name||Methods in Molecular Biology|
Bibliographical noteFunding Information:
This study was in part supported by the grants R01NS046835, R01AG034389, R01NS095215, and NSF grant 1615874.
© 2017, Springer Science+Business Media New York.
- Oligodendrocyte precursor
ASJC Scopus subject areas
- Molecular Biology