Ceramide and S1P signaling in embryonic stem cell differentiation

Guanghu Wang; Stefka D. Spassieva; Erhard Bieberich

doi:10.1007/7651_2017_43

Ceramide and S1P signaling in embryonic stem cell differentiation

Physiology

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

21 Scopus citations

Abstract

Bioactive sphingolipids are important regulators for stem cell survival and differentiation. Most recently, we have coined the term “morphogenetic lipids” for sphingolipids that regulate stem cells during embryonic and postnatal development. The sphingolipid ceramide and its derivative, sphingosine-1-phosphate (S1P), can act synergistically as well as antagonistically on embryonic stem (ES) cell differentiation. We show here simple as well as state-of-the-art methods to analyze sphingolipids in differentiating ES cells and discuss new protocols to use ceramide and S1P analogs for the guided differentiation of mouse ES cells toward neuronal and glial lineage.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	153-171
Number of pages	19
DOIs	https://doi.org/10.1007/7651_2017_43
State	Published - 2018

Publication series

Name	Methods in Molecular Biology
Volume	1697
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© 2017, Springer Science+Business Media New York.

Funding

This study was in part supported by the grants R01NS046835, R01AG034389, R01NS095215, and NSF grant 1615874.

Funders	Funder number
National Science Foundation Arctic Social Science Program	R01NS095215, R01AG034389
National Science Foundation Arctic Social Science Program
Institute of Neurological Disorders and Stroke National Advisory Neurological Disorders and Stroke Council	R01NS046835
Institute of Neurological Disorders and Stroke National Advisory Neurological Disorders and Stroke Council

Keywords

Apoptosis
Ceramide
Neuroprogenitor
Oligodendrocyte precursor
Sphingolipid
Sphingosine-1-phosphate
Teratoma

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/7651_2017_43

Cite this

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AU - Spassieva, Stefka D.

AU - Bieberich, Erhard

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KW - Neuroprogenitor

KW - Oligodendrocyte precursor

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KW - Sphingosine-1-phosphate

KW - Teratoma

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Ceramide and S1P signaling in embryonic stem cell differentiation

Abstract

Publication series

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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