Changes in lipoxygenase isozyme levels during soybean embryo development

David F. Hildebrand, Robert T. Versluys, Glenn B. Collins

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Most soybean (Glycine max Merr.), cultivars accumulate three isozymes of lipoxygenase during embryo development. Studies were conducted to determine the levels of expression and accumulation of the individual lipoxygenase isozymes througout the development of soybean axes and cotyledons. Type I lipoxygenase activity (activity at pH 9.0 indicative of lipoxygenase 1) was not detectable in axes or cotyledons from seeds <6-7 mm in length, but lipoxygenase 1 activity and protein reached high levels at subsequent developmental stages. Type II activity (activity at pH 6.8 indicative of lipoxygenase 2 + 3) was detectable throughout development, with low levels early in development and high levels being present concommitant with lipoxygenase 1. The type II lipoxygenase activity in embryo tissues of seeds <6-7 mm in lenght was apparently not due to6lipoxygenases 2 and/or 3. Levels of all three lipoxygenase isozymes were coordinately regulated during soybean embryo development with higher levels generally expressed in axes than in cotyledons. The expression of these lipoxygenase isozymes appears similar to that of storage proteins and is coincident with the development of germination capability of soybean seeds.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalPlant Science
Volume75
Issue number1
DOIs
StatePublished - 1991

Bibliographical note

Funding Information:
The authors wish to thank Jeap Songlin and Kadum Ali for excellent technical assistance and Dr. Scott Grayburn for help with the photography. The editorial advice of Dr. Tekrony is also greatly appreciated. This work was supported by the Kentucky Agricultural Experiment Station and by Pioneer Hybrid International Seed Co.

Keywords

  • Glycine max
  • differentiation
  • lipid metabolism
  • seeds

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

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