The wild type (Wt) and mutant form of yeast (sphingolipid compensation) genes, SLC1 and SLC1-1, have been shown to have lysophosphatidic acid acyltransferase (LPAT) activities (Nageic et al. in J Biol Chem 269:22156-22163, 1993). Expression of these LPAT genes was reported to increase oil content in transgenic Arabidopsis and Brassica napus. It is of interest to determine if the TAG content increase would also be seen in soybeans. Therefore, the wild type SLC1 was expressed in soybean somatic embryos under the control of seed specific phaseolin promoter. Some transgenic somatic embryos and in both T2 and T3 transgenic seeds showed higher oil contents. Compared to controls, the average increase in triglyceride values went up by 1.5% in transgenic somatic embryos. A maximum of 3.2% increase in seed oil content was observed in a T3 line. Expression of the yeast Wt LPAT gene did not alter the fatty acid composition of the seed oil.
|Number of pages||7|
|State||Published - Oct 2009|
Bibliographical noteFunding Information:
Acknowledgments The authors acknowledge the technical help provided by Wei Chen. The authors also acknowledge the financial support of the United Soybean Board and the support of the Kentucky Agricultural Experiment Station.
- Analytical techniques
- Fatty acid analysis
- Neutral lipid biosynthetic enzymes
- Plant lipid biochemistry
ASJC Scopus subject areas
- Organic Chemistry
- Cell Biology