Enediyne natural products are extremely potent antitumor antibiotics with a remarkable core structure consisting of two acetylenic groups conjugated to a double bond within either a 9- or 10-membered ring. Biosynthesis of this fascinating scaffold is catalyzed in part by an unusual iterative type I polyketide synthase, PKSE, that is shared among all enediyne biosynthetic pathways whose gene clusters have been sequenced to date. The PKSE is unusual in two main respects: (1) it contains an acyl carrier protein (ACP) domain with no sequence homology to any known proteins, and (2) it is self-phosphopantetheinylated by an integrated phosphopantetheinyl transferase (PPTase) domain. The unusual domain architecture and biochemistry of the PKSE hold promise both for the rapid identification of new enediyne natural products and for obtaining fundamental catalytic insights into enediyne biosynthesis. This chapter describes methods for rapid PCR-based classification of conserved enediyne biosynthetic genes, heterologous production of 9-membered PKSE proteins and isolation of the resulting polyene product, and in vitro characterization of the PKSE ACP domain.
|Title of host publication||Complex Enzymes in Microbial Natural Product Biosynthesis, Part B|
|Subtitle of host publication||Polyketides, Aminocoumarins and Carbohydrates|
|Number of pages||16|
|State||Published - 2009|
|Name||Methods in Enzymology|
Bibliographical noteFunding Information:
This work was supported in part by National Institute of Health (NIH) grants CA78747 and CA113297. G.P.H. is the recipient of an Natural Sciences and Engineering Research Council (Canada) (NSERC) postdoctoral fellowship, and S.V.L. is the recipient of an NIH postdoctoral fellowship (CA1059845).
ASJC Scopus subject areas
- Molecular Biology