TY - JOUR
T1 - Characterization and Localization of a Long‐Chain Isoprenyltransferase Activity in Porcine Brain
T2 - Proposed Role in the Biosynthesis of Dolichyl Phosphate
AU - Crick, Dean C.
AU - Rush, Jeffrey S.
AU - Waechter, Charles J.
PY - 1991/10
Y1 - 1991/10
N2 - Pig brain microsomes catalyzed the enzymatic transfer of radiolabeled isoprenyl groups from [1‐14C] isopentenyl pyrophosphate ([1‐MC] I‐P‐P) into long‐chain polyisoprenyl pyrophosphates (Poly‐P‐P) and unidentified neutral lipids. The brain isoprenyltransferase activity synthesizing the Poly‐P‐P (1) required 5 mM Mg2+ and 10 mM vanadate ions for maximal activity; (2) exhibited an apparent Km of 8 μM I‐P‐P; (3) utilized exogenous farnesyl pyrophosphate and two stereoisomers of geranylgeranyl pyrophosphate as substrates; (4) was optimal at pH 8.5; and (S) was stimulated by dithiothreitol. The major products were identified as C90and Q95 allyhic Poly‐P‐P on the basis of the following chemical and chromatographic properties: (1) the intact product cochromatographed with authentic Poly‐P‐P on silica‐gel‐impregnated paper, (2) the major product was converted to a compound chromatographically identical to polyisoprenyl monophosphate (Poly‐P) by alkaline hydrolysis; (3) treatment of the labeled Poly‐P with wheat germ acid phosphatase or mild acid yielded neutral labeled products; (4) the KOH hydrolyzed product coeluted with authentic Poly‐P from lipophilic Sephadex LH‐20; and (5) the labeled lipids produced by enzymatic dephosphorylation had mobilities identical to fully unsaturated polyisoprenols containing 18 (C90) and 19 (Q95) isoprene units when analyzed by reverse‐phase chromatography. When subcellular fractions from rat brain gray matter were compared, the highest specific activity was found in the heavy microsomes. These results demonstrate that brain contains an isoprenyltransferase activity, associated with the rough endoplasmic reticulum, capable of synthesizing long‐chain Poly‐P‐P. The enzymatic reactions by which the Poly‐P‐P intermediate is converted to dolichyl phosphate remain to be elucidated.
AB - Pig brain microsomes catalyzed the enzymatic transfer of radiolabeled isoprenyl groups from [1‐14C] isopentenyl pyrophosphate ([1‐MC] I‐P‐P) into long‐chain polyisoprenyl pyrophosphates (Poly‐P‐P) and unidentified neutral lipids. The brain isoprenyltransferase activity synthesizing the Poly‐P‐P (1) required 5 mM Mg2+ and 10 mM vanadate ions for maximal activity; (2) exhibited an apparent Km of 8 μM I‐P‐P; (3) utilized exogenous farnesyl pyrophosphate and two stereoisomers of geranylgeranyl pyrophosphate as substrates; (4) was optimal at pH 8.5; and (S) was stimulated by dithiothreitol. The major products were identified as C90and Q95 allyhic Poly‐P‐P on the basis of the following chemical and chromatographic properties: (1) the intact product cochromatographed with authentic Poly‐P‐P on silica‐gel‐impregnated paper, (2) the major product was converted to a compound chromatographically identical to polyisoprenyl monophosphate (Poly‐P) by alkaline hydrolysis; (3) treatment of the labeled Poly‐P with wheat germ acid phosphatase or mild acid yielded neutral labeled products; (4) the KOH hydrolyzed product coeluted with authentic Poly‐P from lipophilic Sephadex LH‐20; and (5) the labeled lipids produced by enzymatic dephosphorylation had mobilities identical to fully unsaturated polyisoprenols containing 18 (C90) and 19 (Q95) isoprene units when analyzed by reverse‐phase chromatography. When subcellular fractions from rat brain gray matter were compared, the highest specific activity was found in the heavy microsomes. These results demonstrate that brain contains an isoprenyltransferase activity, associated with the rough endoplasmic reticulum, capable of synthesizing long‐chain Poly‐P‐P. The enzymatic reactions by which the Poly‐P‐P intermediate is converted to dolichyl phosphate remain to be elucidated.
KW - Dolichyl phosphate
KW - Endoplasmic reticulum
KW - Isoprenyltransferase
KW - Polyisoprenyl pyrophosphate
KW - bopentenyl pyrophosphate
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U2 - 10.1111/j.1471-4159.1991.tb08301.x
DO - 10.1111/j.1471-4159.1991.tb08301.x
M3 - Article
C2 - 1895109
AN - SCOPUS:0025994322
SN - 0022-3042
VL - 57
SP - 1354
EP - 1362
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 4
ER -