Characterization and sequencing of lithium cationized β-O-4 lignin oligomers using higher-energy collisional dissociation mass spectrometry

Shardrack O. Asare, Fan Huang, Bert C. Lynn

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

The prospect of developing new bio-based products from lignin partially depends on the development of effective analytical techniques to characterize the end products from lignin degradation experiments. To date, the most utilized mass spectrometric technique for characterizing lignin oligomers has been negative ion mass spectrometry. Positive ion mass spectrometry remains a relatively unexplored approach for lignin sequencing. Here we report on the sequencing of lignin oligomers using lithium cationization positive ion electrospray followed by higher-energy collisional dissociation (HCD) tandem mass spectrometry on a high-resolution accurate-mass Orbitrap mass spectrometer. Thirteen synthetic lignin model oligomers containing β-O-4 and 4-O-α linkages were analyzed and sequenced based on their HCD tandem mass spectrometry fragmentation patterns. Proposed sequence-specific fragmentation pathways are provided. The method presented in this study provides a potential application of lithium cationization tandem mass spectrometry for the analysis of lignin degradation products.

Original languageEnglish
Pages (from-to)104-114
Number of pages11
JournalAnalytica Chimica Acta
Volume1047
DOIs
StatePublished - Jan 24 2019

Bibliographical note

Publisher Copyright:
© 2018 Elsevier B.V.

Keywords

  • HCD
  • Lignin
  • Lithium adduct
  • Positive mode electrospray ionization
  • Sequencing
  • Tandem mass spectrometry

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Fingerprint

Dive into the research topics of 'Characterization and sequencing of lithium cationized β-O-4 lignin oligomers using higher-energy collisional dissociation mass spectrometry'. Together they form a unique fingerprint.

Cite this