In the present studies we have evaluated the optimal operating conditions for the Hamilton-Thorn HTM-2000 computerized semen analyzer (Hamilton-Thorn, Danvers, MA). The best reproducibility in measurement of sperm concentration was obtained using 20 frames acquired at 19 frames/s. The measurement of sperm concentration was not adversely affected by the number of fields analyzed. The intrasample and intersample co-efficients of variation for sperm concentration were 9.5% and 25.5%; sperm motility, 18.4% and 28.9%; lateral head displacement, 16.5% and 19.9%; path velocity, 6.8% and 13.9%; progressive velocity, 4.5% and 9.9%; and linear index, 2.5% and 4.2%; respectively. These differences suggest that sampling error has a significant influence on the reliability of sperm evaluation. The precision and rapidity of the HTM-2000 compares favorably with data previously reported from other systems available for clinical semen analysis.
|Number of pages||8|
|Journal||Fertility and Sterility|
|State||Published - 1989|
Bibliographical noteFunding Information:
Acknowledgments. We express our sincere thanks to Mr. Scott Estes and Ms. Terri Clarke for their expert technical assistance. Purchase of the Hamilton HTM-2000 semen analyzer was supported by the University of Kentucky Major Research Instrumentation Bond Program (Bond no. L-083-7H500).
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynecology