Characterization of a fourth streptococcal pyrogenic exotoxin (SPE D)

Ray A. McMillian; Timothy A. Bloomster; Abdul M. Saeed; Kristina L. Henderson; Nancy E. Zinn; Roy Abernathy; Dennis W. Watson; Richard N. Greenberg

doi:10.1111/j.1574-6968.1987.tb02308.x

Characterization of a fourth streptococcal pyrogenic exotoxin (SPE D)

Ray A. McMillian, Timothy A. Bloomster, Abdul M. Saeed, Kristina L. Henderson, Nancy E. Zinn, Roy Abernathy, Dennis W. Watson, Richard N. Greenberg

Internal Medicine

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

A fourth streptococcal pyrogenic exotoxin (SPE D) was isolated from a pathogenic strain of group A streptococci cultured from a patient with a fatal case of streptococcal fasciitis associated with hypocalcemia and 'toxic' syndrome. SPE D was partially purified by differential solubility in ethanol and acetate-buffered saline and thin-layer isoelectric focusing. SPE D was antigenically distinct from the other streptococcal exotoxins SPE A, SPE B and SPE C. The isoelectric point of this exotoxin (4.6) was lower than those of the other SPEs. The M_r was estimated to be 13 000, and the biological activities were similar to the other exotoxins. SPE D reacted synergistically with SPE C (also present in this strain of streptococcus) to enhance the lethal effect of endotoxin in rabbits. The role of SPEs in the pathogenicity of this organism is discussed.

Original language	English
Pages (from-to)	317-322
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	44
Issue number	3
DOIs	https://doi.org/10.1111/j.1574-6968.1987.tb02308.x
State	Published - Nov 1987

Bibliographical note

Funding Information:
We appreciate the secretarial support of Mrs. Sue Stevens. This work was supported in part by the Institute of Medical Education and Research, Saint Louis, MO, and the Cancer Research Institute, New York, NY.

Funding

We appreciate the secretarial support of Mrs. Sue Stevens. This work was supported in part by the Institute of Medical Education and Research, Saint Louis, MO, and the Cancer Research Institute, New York, NY.

Funders	Funder number
Postgraduate Institute of Medical Education and Research
Children's Cancer Research Institute, Vienna
Saint Louis University

Keywords

(Fasciitis)
(Hypocalcemia)
Group A streptococci
SPE-A
SPE-B
SPE-C
SPE-D
Streptococcal pyrogenic exotoxin

ASJC Scopus subject areas

General Medicine

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title = "Characterization of a fourth streptococcal pyrogenic exotoxin (SPE D)",

abstract = "A fourth streptococcal pyrogenic exotoxin (SPE D) was isolated from a pathogenic strain of group A streptococci cultured from a patient with a fatal case of streptococcal fasciitis associated with hypocalcemia and 'toxic' syndrome. SPE D was partially purified by differential solubility in ethanol and acetate-buffered saline and thin-layer isoelectric focusing. SPE D was antigenically distinct from the other streptococcal exotoxins SPE A, SPE B and SPE C. The isoelectric point of this exotoxin (4.6) was lower than those of the other SPEs. The Mr was estimated to be 13 000, and the biological activities were similar to the other exotoxins. SPE D reacted synergistically with SPE C (also present in this strain of streptococcus) to enhance the lethal effect of endotoxin in rabbits. The role of SPEs in the pathogenicity of this organism is discussed.",

keywords = "(Fasciitis), (Hypocalcemia), Group A streptococci, SPE-A, SPE-B, SPE-C, SPE-D, Streptococcal pyrogenic exotoxin",

author = "McMillian, {Ray A.} and Bloomster, {Timothy A.} and Saeed, {Abdul M.} and Henderson, {Kristina L.} and Zinn, {Nancy E.} and Roy Abernathy and Watson, {Dennis W.} and Greenberg, {Richard N.}",

note = "Funding Information: We appreciate the secretarial support of Mrs. Sue Stevens. This work was supported in part by the Institute of Medical Education and Research, Saint Louis, MO, and the Cancer Research Institute, New York, NY.",

year = "1987",

month = nov,

doi = "10.1111/j.1574-6968.1987.tb02308.x",

language = "English",

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T1 - Characterization of a fourth streptococcal pyrogenic exotoxin (SPE D)

AU - McMillian, Ray A.

AU - Bloomster, Timothy A.

AU - Saeed, Abdul M.

AU - Henderson, Kristina L.

AU - Zinn, Nancy E.

AU - Abernathy, Roy

AU - Watson, Dennis W.

AU - Greenberg, Richard N.

N1 - Funding Information: We appreciate the secretarial support of Mrs. Sue Stevens. This work was supported in part by the Institute of Medical Education and Research, Saint Louis, MO, and the Cancer Research Institute, New York, NY.

PY - 1987/11

Y1 - 1987/11

N2 - A fourth streptococcal pyrogenic exotoxin (SPE D) was isolated from a pathogenic strain of group A streptococci cultured from a patient with a fatal case of streptococcal fasciitis associated with hypocalcemia and 'toxic' syndrome. SPE D was partially purified by differential solubility in ethanol and acetate-buffered saline and thin-layer isoelectric focusing. SPE D was antigenically distinct from the other streptococcal exotoxins SPE A, SPE B and SPE C. The isoelectric point of this exotoxin (4.6) was lower than those of the other SPEs. The Mr was estimated to be 13 000, and the biological activities were similar to the other exotoxins. SPE D reacted synergistically with SPE C (also present in this strain of streptococcus) to enhance the lethal effect of endotoxin in rabbits. The role of SPEs in the pathogenicity of this organism is discussed.

AB - A fourth streptococcal pyrogenic exotoxin (SPE D) was isolated from a pathogenic strain of group A streptococci cultured from a patient with a fatal case of streptococcal fasciitis associated with hypocalcemia and 'toxic' syndrome. SPE D was partially purified by differential solubility in ethanol and acetate-buffered saline and thin-layer isoelectric focusing. SPE D was antigenically distinct from the other streptococcal exotoxins SPE A, SPE B and SPE C. The isoelectric point of this exotoxin (4.6) was lower than those of the other SPEs. The Mr was estimated to be 13 000, and the biological activities were similar to the other exotoxins. SPE D reacted synergistically with SPE C (also present in this strain of streptococcus) to enhance the lethal effect of endotoxin in rabbits. The role of SPEs in the pathogenicity of this organism is discussed.

KW - (Fasciitis)

KW - (Hypocalcemia)

KW - Group A streptococci

KW - SPE-A

KW - SPE-B

KW - SPE-C

KW - SPE-D

KW - Streptococcal pyrogenic exotoxin

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SN - 0378-1097

VL - 44

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EP - 322

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

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ER -

Characterization of a fourth streptococcal pyrogenic exotoxin (SPE D)

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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