Characterization of a fourth streptococcal pyrogenic exotoxin (SPE D)

Ray A. McMillian, Timothy A. Bloomster, Abdul M. Saeed, Kristina L. Henderson, Nancy E. Zinn, Roy Abernathy, Dennis W. Watson, Richard N. Greenberg

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


A fourth streptococcal pyrogenic exotoxin (SPE D) was isolated from a pathogenic strain of group A streptococci cultured from a patient with a fatal case of streptococcal fasciitis associated with hypocalcemia and 'toxic' syndrome. SPE D was partially purified by differential solubility in ethanol and acetate-buffered saline and thin-layer isoelectric focusing. SPE D was antigenically distinct from the other streptococcal exotoxins SPE A, SPE B and SPE C. The isoelectric point of this exotoxin (4.6) was lower than those of the other SPEs. The Mr was estimated to be 13 000, and the biological activities were similar to the other exotoxins. SPE D reacted synergistically with SPE C (also present in this strain of streptococcus) to enhance the lethal effect of endotoxin in rabbits. The role of SPEs in the pathogenicity of this organism is discussed.

Original languageEnglish
Pages (from-to)317-322
Number of pages6
JournalFEMS Microbiology Letters
Issue number3
StatePublished - Nov 1987

Bibliographical note

Funding Information:
We appreciate the secretarial support of Mrs. Sue Stevens. This work was supported in part by the Institute of Medical Education and Research, Saint Louis, MO, and the Cancer Research Institute, New York, NY.


  • (Fasciitis)
  • (Hypocalcemia)
  • Group A streptococci
  • SPE-A
  • SPE-B
  • SPE-C
  • SPE-D
  • Streptococcal pyrogenic exotoxin

ASJC Scopus subject areas

  • General Medicine


Dive into the research topics of 'Characterization of a fourth streptococcal pyrogenic exotoxin (SPE D)'. Together they form a unique fingerprint.

Cite this