Characterization of a monoclonal antibody against CREPT, a novel protein highly expressed in tumors

Fangli Ren, Ruoke Wang, Yanquan Zhang, Chunxiao Liu, Yinyin Wang, Jim Hu, Linqi Zhang, Zhijie Chang

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


CREPT (cell-cycle related and expression-elevated protein in tumor), a novel gene also called RPRD1B and C20ORF77, was recently identified to promote tumorigenesis through up-regulation of the expression of genes related to cell cycle. The previous study demonstrated that CREPT is highly expressed in a variety of tumors and enhances the expression of Cyclin D1 by promoting the formation of a chromatin loop. To study the correlation of CREPT expression with clinical factors in different tumors, we generated a monoclonal antibody (3E10) using purified recombinant human GST-CREPT protein as an antigen. In this study, we characterized the specificity of the monoclonal antibody and cloned the gene encoding the antibody for preparation of industrial production. Our results showed that the monoclonal antibody 3E10 was sensitive and specific to recognize human endogenous CREPT protein. We have mapped the epitope of the antibody and cloned the variable region sequence of the gene encoding the antibody. We confirmed that the cloned gene produced an equivalent antibody as that produced by the original hybridoma. This study provided a basis for large-scale production of the CREPT antibody, which will be useful for the study of the role of CREPT in different tumors.

Original languageEnglish
Pages (from-to)401-408
Number of pages8
JournalMonoclonal Antibodies in Immunodiagnosis and Immunotherapy
Issue number6
StatePublished - Dec 1 2014

Bibliographical note

Publisher Copyright:
© Copyright 2014, Mary Ann Liebert, Inc.

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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