Characterization of a new aedes albopictus (Diptera: Culicidae)-Wolbachia pipientis (Rickettsiales: Rickettsiaceae) symbiotic association generated by artificial transfer of the wPip strain from culex pipiens (Diptera: Culicidae)

Maurizio Calvitti, Riccardo Moretti, Elena Lampazzi, Romeo Bellini, Stephen L. Dobson

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

Wolbachia is a maternally inherited endosymbiont inducing various effects in insects and other invertebrate hosts that facilitate the invasion of nave host populations. One of the effects is a form of sterility known as cytoplasmic incompatibility (CI) through which females are effectively sterilized when they mate with males harboring a different Wolbachia strain. The repeated mass release of cytoplasmically incompatible males can be a tool to suppress insect populations. Here, we attempt to infect an Aedes albopictus (Skuse) (Diptera: Culicidae) strain, artificially deprived of the natural Wolbachia infection, with a new Wolbachia strain from Culex pipiens (L.) (Diptera: Culicidae). Further experiments were designed to study the effects of the new infection on Ae. albopictus fitness and evaluate key parameters that affect infection dynamics, including CI level and maternal inheritance. Using embryonic microinjection, the new Wolbachia strain was successfully established in Ae. albopictus. Crosses demonstrated a pattern of bidirectional CI between naturally infected and transinfected individuals. Specifically, egg hatch was essentially absent (i.e., CI was very high) in all crosses between the transinfected males and females with a different infection status. Furthermore, naturally infected Ae. albopictus males were incompatible with the transinfected females. Maternal inheritance was close to 100%. Moreover, the new infection did not affect immature and adult survivorship, but it significantly reduced female fecundity and egg hatch rate. The results are discussed in relation to the potential use of the new Ae. albopictusWolbachia symbiotic association as a suitable system for the study and development of CI-based strategies for suppressing populations of this important pest and disease vector.

Original languageEnglish
Pages (from-to)179-187
Number of pages9
JournalJournal of Medical Entomology
Volume47
Issue number2
DOIs
StatePublished - Feb 2010

Keywords

  • Aedes albopictus
  • Cytoplasmic incompatibility
  • Genetic control
  • Transinfection
  • Wolbachia pipientis

ASJC Scopus subject areas

  • Parasitology
  • General Veterinary
  • Insect Science
  • Infectious Diseases

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