Characterization of a novel plant poly(A) polymerase

Jaydip Das Gupta, Qingshun Li, A. Brian Thomson, Arthur G. Hunt

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


We have purified and characterized poly(A) polymerases (PAPs) from Pisum sativum, Brassica juncea, and Zea mays. Through chromatography on DEAE-Sepharose and heparin-Sepharose, these PAPs copurified as a single enzyme along with RNPs that could provide RNA substrates for the enzyme. More extensive purification by chromatography on MonoQ resulted in the resolution of the PAPs into as many as three fractions. One of these (PAP-I) contained a 43-kDa polypeptide immunologically related to the yeast PAP, and two others (PAP-II and PAP-III) contained RNAs that could serve as substrates for polyadenylation. These fractions by themselves possessed little PAP activity, but mixtures containing combinations of these displayed substantial activity. Similar PAP factors (PAP-I and PAP-III) were identified after fractionation of extracts prepared from Brassica juncea and Zea mays. The factors from one plant were completely interchangeable with those from different plants. We conclude that the poly(A) polymerases present in vegetative plant tissues consist of more than one component. In this respect, they are substantially different from other reported plant, mammalian, and yeast PAPs.

Original languageEnglish
Pages (from-to)215-226
Number of pages12
JournalPlant Science
Issue number2
StatePublished - Sep 15 1995


  • Posttranscriptional control
  • RNA processing
  • mRNA Polyadenylation

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science


Dive into the research topics of 'Characterization of a novel plant poly(A) polymerase'. Together they form a unique fingerprint.

Cite this