Characterization of bovine ileal epithelial cell line for lectin binding, susceptibility to enteric pathogens, and TLR mediated immune responses

Pratik Katwal; Tirth Uprety; Faten Okda; Linto Antony; Milton Thomas; Christopher Chase; Diego G. Diel; Eric Nelson; Alan Young; Feng Li; Joy Scaria; Radhey S. Kaushik

doi:10.1016/j.cimid.2020.101581

Characterization of bovine ileal epithelial cell line for lectin binding, susceptibility to enteric pathogens, and TLR mediated immune responses

Pratik Katwal, Tirth Uprety, Faten Okda, Linto Antony, Milton Thomas, Christopher Chase, Diego G. Diel, Eric Nelson, Alan Young, Feng Li, Joy Scaria, Radhey S. Kaushik

Veterinary Science

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

In this study, primary and immortalized bovine intestinal epithelial cells (BIECs) were characterized for the expression of surface carbohydrate moieties. Primary BIEC-c4 cells showed staining greater than 90 % for 16 lectins but less than 50 % staining for four lectins. Immortalized BIECs showed significantly different lectin binding profile for few lectins compared to BIEC-c4 cells. BIEC-c4 cells were studied for infectivity to E. coli, Salmonella enterica, bovine rotavirus, bovine coronavirus, and bovine viral diarrhea virus. Bovine strain E. coli B41 adhered to BIEC-c4 cells and Salmonella strains S. Dublin and S. Mbandaka showed strong cell invasion. BIEC-c4 cells were susceptible to bovine rotavirus. LPS stimulation upregulated IL-10, IL-8, and IL-6 expression and Poly I:C upregulated TLR 8 and TLR 9 expression. This study provides important knowledge on the glycoconjugate expression profile of primary and immortalized BIECs and infectivity and immune responses of primary BIECs to bacterial and viral pathogens or ligands.

Original language	English
Article number	101581
Journal	Comparative Immunology, Microbiology and Infectious Diseases
Volume	74
DOIs	https://doi.org/10.1016/j.cimid.2020.101581
State	Published - Feb 2021

Bibliographical note

Funding Information:
Radhey S. Kaushik, Pratik Katwal, Tirth Uprety, and Milton Thomas, and this research project were funded and supported by USDA , NIFA , SDSU Agricultural Experiment Station Hatch grants # SD00H326-09 , and SD00H547-15 . This study was also funded by USDA/CSREES through project title JDIP: Johne’s Disease Integrated Program in Research, Education, and Extension , grant number # 2004-35605-14243 ; Sub-award No. Q6286224171. We acknowledge use of the SDSU-FGCF supported in part by NSF/EPSCoR Grant No. 0091948 and by the State of South Dakota.

Publisher Copyright:
© 2020 Elsevier Ltd

Keywords

Bovine coronavirus
Bovine intestinal epithelial cells
Bovine rotavirus
Bovine viral diarrhea virus
Cytokines
Enterotoxigenic E. coli
Lectin binding profile
Salmonella
Toll-like receptors

ASJC Scopus subject areas

Immunology and Allergy
Microbiology
Immunology
Veterinary (all)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.cimid.2020.101581

Cite this

Katwal, P., Uprety, T., Okda, F., Antony, L., Thomas, M., Chase, C., Diel, D. G., Nelson, E., Young, A., Li, F., Scaria, J., & Kaushik, R. S. (2021). Characterization of bovine ileal epithelial cell line for lectin binding, susceptibility to enteric pathogens, and TLR mediated immune responses. Comparative Immunology, Microbiology and Infectious Diseases, 74, [101581]. https://doi.org/10.1016/j.cimid.2020.101581

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abstract = "In this study, primary and immortalized bovine intestinal epithelial cells (BIECs) were characterized for the expression of surface carbohydrate moieties. Primary BIEC-c4 cells showed staining greater than 90 % for 16 lectins but less than 50 % staining for four lectins. Immortalized BIECs showed significantly different lectin binding profile for few lectins compared to BIEC-c4 cells. BIEC-c4 cells were studied for infectivity to E. coli, Salmonella enterica, bovine rotavirus, bovine coronavirus, and bovine viral diarrhea virus. Bovine strain E. coli B41 adhered to BIEC-c4 cells and Salmonella strains S. Dublin and S. Mbandaka showed strong cell invasion. BIEC-c4 cells were susceptible to bovine rotavirus. LPS stimulation upregulated IL-10, IL-8, and IL-6 expression and Poly I:C upregulated TLR 8 and TLR 9 expression. This study provides important knowledge on the glycoconjugate expression profile of primary and immortalized BIECs and infectivity and immune responses of primary BIECs to bacterial and viral pathogens or ligands.",

keywords = "Bovine coronavirus, Bovine intestinal epithelial cells, Bovine rotavirus, Bovine viral diarrhea virus, Cytokines, Enterotoxigenic E. coli, Lectin binding profile, Salmonella, Toll-like receptors",

author = "Pratik Katwal and Tirth Uprety and Faten Okda and Linto Antony and Milton Thomas and Christopher Chase and Diel, {Diego G.} and Eric Nelson and Alan Young and Feng Li and Joy Scaria and Kaushik, {Radhey S.}",

note = "Funding Information: Radhey S. Kaushik, Pratik Katwal, Tirth Uprety, and Milton Thomas, and this research project were funded and supported by USDA , NIFA , SDSU Agricultural Experiment Station Hatch grants # SD00H326-09 , and SD00H547-15 . This study was also funded by USDA/CSREES through project title JDIP: Johne{\textquoteright}s Disease Integrated Program in Research, Education, and Extension , grant number # 2004-35605-14243 ; Sub-award No. Q6286224171. We acknowledge use of the SDSU-FGCF supported in part by NSF/EPSCoR Grant No. 0091948 and by the State of South Dakota. Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",

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Katwal, P, Uprety, T, Okda, F, Antony, L, Thomas, M, Chase, C, Diel, DG, Nelson, E, Young, A, Li, F, Scaria, J & Kaushik, RS 2021, 'Characterization of bovine ileal epithelial cell line for lectin binding, susceptibility to enteric pathogens, and TLR mediated immune responses', Comparative Immunology, Microbiology and Infectious Diseases, vol. 74, 101581. https://doi.org/10.1016/j.cimid.2020.101581

TY - JOUR

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AU - Katwal, Pratik

AU - Uprety, Tirth

AU - Okda, Faten

AU - Antony, Linto

AU - Thomas, Milton

AU - Chase, Christopher

AU - Diel, Diego G.

AU - Nelson, Eric

AU - Young, Alan

AU - Li, Feng

AU - Scaria, Joy

AU - Kaushik, Radhey S.

N1 - Funding Information: Radhey S. Kaushik, Pratik Katwal, Tirth Uprety, and Milton Thomas, and this research project were funded and supported by USDA , NIFA , SDSU Agricultural Experiment Station Hatch grants # SD00H326-09 , and SD00H547-15 . This study was also funded by USDA/CSREES through project title JDIP: Johne’s Disease Integrated Program in Research, Education, and Extension , grant number # 2004-35605-14243 ; Sub-award No. Q6286224171. We acknowledge use of the SDSU-FGCF supported in part by NSF/EPSCoR Grant No. 0091948 and by the State of South Dakota. Publisher Copyright: © 2020 Elsevier Ltd

PY - 2021/2

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N2 - In this study, primary and immortalized bovine intestinal epithelial cells (BIECs) were characterized for the expression of surface carbohydrate moieties. Primary BIEC-c4 cells showed staining greater than 90 % for 16 lectins but less than 50 % staining for four lectins. Immortalized BIECs showed significantly different lectin binding profile for few lectins compared to BIEC-c4 cells. BIEC-c4 cells were studied for infectivity to E. coli, Salmonella enterica, bovine rotavirus, bovine coronavirus, and bovine viral diarrhea virus. Bovine strain E. coli B41 adhered to BIEC-c4 cells and Salmonella strains S. Dublin and S. Mbandaka showed strong cell invasion. BIEC-c4 cells were susceptible to bovine rotavirus. LPS stimulation upregulated IL-10, IL-8, and IL-6 expression and Poly I:C upregulated TLR 8 and TLR 9 expression. This study provides important knowledge on the glycoconjugate expression profile of primary and immortalized BIECs and infectivity and immune responses of primary BIECs to bacterial and viral pathogens or ligands.

AB - In this study, primary and immortalized bovine intestinal epithelial cells (BIECs) were characterized for the expression of surface carbohydrate moieties. Primary BIEC-c4 cells showed staining greater than 90 % for 16 lectins but less than 50 % staining for four lectins. Immortalized BIECs showed significantly different lectin binding profile for few lectins compared to BIEC-c4 cells. BIEC-c4 cells were studied for infectivity to E. coli, Salmonella enterica, bovine rotavirus, bovine coronavirus, and bovine viral diarrhea virus. Bovine strain E. coli B41 adhered to BIEC-c4 cells and Salmonella strains S. Dublin and S. Mbandaka showed strong cell invasion. BIEC-c4 cells were susceptible to bovine rotavirus. LPS stimulation upregulated IL-10, IL-8, and IL-6 expression and Poly I:C upregulated TLR 8 and TLR 9 expression. This study provides important knowledge on the glycoconjugate expression profile of primary and immortalized BIECs and infectivity and immune responses of primary BIECs to bacterial and viral pathogens or ligands.

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KW - Bovine intestinal epithelial cells

KW - Bovine rotavirus

KW - Bovine viral diarrhea virus

KW - Cytokines

KW - Enterotoxigenic E. coli

KW - Lectin binding profile

KW - Salmonella

KW - Toll-like receptors

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DO - 10.1016/j.cimid.2020.101581

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ER -

Characterization of bovine ileal epithelial cell line for lectin binding, susceptibility to enteric pathogens, and TLR mediated immune responses

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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