Abstract
The emergence of viruses in Coffee (Coffea arabica and Coffea canephora), the most widely traded agricultural commodity in the world, is of critical concern. The RNA1 (6552. nt) of Coffee ringspot virus is organized into five open reading frames (ORFs) capable of encoding the viral nucleocapsid (ORF1p), phosphoprotein (ORF2p), putative cell-to-cell movement protein (ORF3p), matrix protein (ORF4p) and glycoprotein (ORF5p). Each ORF is separated by a conserved intergenic junction. RNA2 (5945. nt), which completes the bipartite genome, encodes a single protein (ORF6p) with homology to RNA-dependent RNA polymerases. Phylogenetic analysis of L protein sequences firmly establishes CoRSV as a member of the recently proposed Dichorhavirus genus. Predictive algorithms, in planta protein expression, and a yeast-based nuclear import assay were used to determine the nucleophillic character of five CoRSV proteins. Finally, the temperature-dependent ability of CoRSV to establish systemic infections in an initially local lesion host was quantified.
| Original language | English |
|---|---|
| Pages (from-to) | 385-396 |
| Number of pages | 12 |
| Journal | Virology |
| Volume | 464-465 |
| Issue number | 1 |
| DOIs | |
| State | Published - Sep 2014 |
Bibliographical note
Funding Information:We thank the CNPq , CAPES , FAPEMIG , and NSF funding agencies for providing research grants to M.G. and A.F. We would also like to thank the farmers and Cooperative workers who graciously provided access to their farms in support of this research (CAPES Project No. A009, 1901133, and NSF-IOS-0749519). We wish to thank John Shaw and David Smith for critical reading of the manuscript prior to submission. Finally we wish to thank David Zaitlin at the Kentucky Tobacco Research & Development Center for providing access to growth chambers critical to experiments conducted for this manuscript.
Funding
We thank the CNPq , CAPES , FAPEMIG , and NSF funding agencies for providing research grants to M.G. and A.F. We would also like to thank the farmers and Cooperative workers who graciously provided access to their farms in support of this research (CAPES Project No. A009, 1901133, and NSF-IOS-0749519). We wish to thank John Shaw and David Smith for critical reading of the manuscript prior to submission. Finally we wish to thank David Zaitlin at the Kentucky Tobacco Research & Development Center for providing access to growth chambers critical to experiments conducted for this manuscript.
| Funders | Funder number |
|---|---|
| Coordenação de Aperfeiçoamento de Pessoal de Nível Superior | |
| Conselho Nacional de Desenvolvimento Científico e Tecnológico | |
| Fundação de Amparo à Pesquisa do Estado de Minas Gerais | A009, NSF-IOS-0749519, 1901133 |
Keywords
- Brazil
- Climate change
- Dichorhavirus
- Negative-strand RNA virus
- Protein localization
- Rhabdovirus
- Virus emergence
ASJC Scopus subject areas
- Virology
