The characterization of TDP-α-d-glucose dehydrogenase (AtmS8), TDP-α-d-glucuronic acid decarboxylase (AtmS9), and TDP-4-keto-α-d-xylose 2,3-dehydratase (AtmS14), involved in Actinomadura melliaura AT2433 aminodideoxypentose biosynthesis, is reported. This study provides the first biochemical evidence that both deoxypentose and deoxyhexose biosynthetic pathways share common strategies for sugar 2,3-dehydration/reduction and implicates the sugar nucleotide base specificity of AtmS14 as a potential mechanism for sugar nucleotide commitment to secondary metabolism. In addition, a re-evaluation of the AtmS9 homologue involved in calicheamicin aminodeoxypentose biosynthesis (CalS9) reveals that CalS9 catalyzes UDP-4-keto-α-d-xylose as the predominant product, rather than UDP-α-d-xylose as previously reported. Cumulatively, this work provides additional fundamental insights regarding the biosynthesis of novel pentoses attached to complex bacterial secondary metabolites.
|Number of pages||2|
|State||Published - Oct 1 2015|
Bibliographical notePublisher Copyright:
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
- natural products
- sugar nucleotides
ASJC Scopus subject areas
- Molecular Medicine
- Molecular Biology
- Organic Chemistry