Characterization of elicitor-induction of sesquiterpene cyclase activity in potato tuber tissue

Michael N. Zook; Joseph Chappell; Joseph A. Kúc

doi:10.1016/0031-9422(92)83703-2

Characterization of elicitor-induction of sesquiterpene cyclase activity in potato tuber tissue

Michael N. Zook, Joseph Chappell, Joseph A. Kúc

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The olefin reaction products of the potato and tobacco sesquiterpene cyclases were partially separated from each other by argenation chromatography. Arachidonic acid-elicited potato tuber discs or cellulase-elicited tobacco cell suspension cultures metabolized the olefin reaction products of potato and tobacco sesquiterpene cyclases into different sets of compounds. These results provide evidence that the two olefin cyclase products are chemically different. The K_m of the potato sesquiterpene cyclase for farnesyl diphosphate was 7μM and 1.0 mM for magnesium chloride. Inorganic diphosphate inhibited enzyme activity with an ID₅₀ of approximately 4 mM.

Original language	English
Pages (from-to)	3441-3445
Number of pages	5
Journal	Phytochemistry
Volume	31
Issue number	10
DOIs	https://doi.org/10.1016/0031-9422(92)83703-2
State	Published - Oct 1992

Keywords

5-epi-aristolochene
Solanaceae
Solanum tuberosum
biosynthesis
rishitin
sesquiterpene cyclase.
sesquiterpenoids

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Plant Science
Horticulture

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10.1016/0031-9422(92)83703-2

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title = "Characterization of elicitor-induction of sesquiterpene cyclase activity in potato tuber tissue",

abstract = "The olefin reaction products of the potato and tobacco sesquiterpene cyclases were partially separated from each other by argenation chromatography. Arachidonic acid-elicited potato tuber discs or cellulase-elicited tobacco cell suspension cultures metabolized the olefin reaction products of potato and tobacco sesquiterpene cyclases into different sets of compounds. These results provide evidence that the two olefin cyclase products are chemically different. The Km of the potato sesquiterpene cyclase for farnesyl diphosphate was 7μM and 1.0 mM for magnesium chloride. Inorganic diphosphate inhibited enzyme activity with an ID50 of approximately 4 mM.",

keywords = "5-epi-aristolochene, Solanaceae, Solanum tuberosum, biosynthesis, rishitin, sesquiterpene cyclase., sesquiterpenoids",

author = "Zook, {Michael N.} and Joseph Chappell and K{\'u}c, {Joseph A.}",

year = "1992",

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doi = "10.1016/0031-9422(92)83703-2",

language = "English",

volume = "31",

pages = "3441--3445",

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T1 - Characterization of elicitor-induction of sesquiterpene cyclase activity in potato tuber tissue

AU - Zook, Michael N.

AU - Chappell, Joseph

AU - Kúc, Joseph A.

PY - 1992/10

Y1 - 1992/10

N2 - The olefin reaction products of the potato and tobacco sesquiterpene cyclases were partially separated from each other by argenation chromatography. Arachidonic acid-elicited potato tuber discs or cellulase-elicited tobacco cell suspension cultures metabolized the olefin reaction products of potato and tobacco sesquiterpene cyclases into different sets of compounds. These results provide evidence that the two olefin cyclase products are chemically different. The Km of the potato sesquiterpene cyclase for farnesyl diphosphate was 7μM and 1.0 mM for magnesium chloride. Inorganic diphosphate inhibited enzyme activity with an ID50 of approximately 4 mM.

AB - The olefin reaction products of the potato and tobacco sesquiterpene cyclases were partially separated from each other by argenation chromatography. Arachidonic acid-elicited potato tuber discs or cellulase-elicited tobacco cell suspension cultures metabolized the olefin reaction products of potato and tobacco sesquiterpene cyclases into different sets of compounds. These results provide evidence that the two olefin cyclase products are chemically different. The Km of the potato sesquiterpene cyclase for farnesyl diphosphate was 7μM and 1.0 mM for magnesium chloride. Inorganic diphosphate inhibited enzyme activity with an ID50 of approximately 4 mM.

KW - 5-epi-aristolochene

KW - Solanaceae

KW - Solanum tuberosum

KW - biosynthesis

KW - rishitin

KW - sesquiterpene cyclase.

KW - sesquiterpenoids

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JO - Phytochemistry

JF - Phytochemistry

IS - 10

ER -

Characterization of elicitor-induction of sesquiterpene cyclase activity in potato tuber tissue

Abstract

Keywords

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