Characterization of elicitor-induction of sesquiterpene cyclase activity in potato tuber tissue

Michael N. Zook, Joseph Chappell, Joseph A. Kúc

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The olefin reaction products of the potato and tobacco sesquiterpene cyclases were partially separated from each other by argenation chromatography. Arachidonic acid-elicited potato tuber discs or cellulase-elicited tobacco cell suspension cultures metabolized the olefin reaction products of potato and tobacco sesquiterpene cyclases into different sets of compounds. These results provide evidence that the two olefin cyclase products are chemically different. The Km of the potato sesquiterpene cyclase for farnesyl diphosphate was 7μM and 1.0 mM for magnesium chloride. Inorganic diphosphate inhibited enzyme activity with an ID50 of approximately 4 mM.

Original languageEnglish
Pages (from-to)3441-3445
Number of pages5
JournalPhytochemistry
Volume31
Issue number10
DOIs
StatePublished - Oct 1992

Keywords

  • 5-epi-aristolochene
  • biosynthesis
  • rishitin
  • sesquiterpene cyclase.
  • sesquiterpenoids
  • Solanaceae
  • Solanum tuberosum

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Horticulture

Fingerprint

Dive into the research topics of 'Characterization of elicitor-induction of sesquiterpene cyclase activity in potato tuber tissue'. Together they form a unique fingerprint.

Cite this