Characterization of equine and other vertebrate TLR3, TLR7, and TLR8 genes

Natalia M. Astakhova, Andrey A. Perelygin, Andrey A. Zharkikh, Teri L. Lear, Stephen J. Coleman, James N. MacLeod, Margo A. Brinton

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Toll-like receptors 3, 7, and 8 (TLR3, TLR7, and TLR8) were studied in the genomes of the domestic horse and several other mammals. The messenger RNA sequences and exon/intron structures of these TLR genes were determined. An equine bacterial artificial chromosome clone containing the TLR3 gene was assigned by fluorescent in situ hybridization to the horse chromosomal location ECA27q16-q17 and this map location was confirmed using an equine radiation hybrid panel. Direct sequencing revealed 13 single-nucleotide polymorphisms in the coding regions of the equine TLR 3, 7, and 8 genes. Of these polymorphisms, 12 were not previously reported. The allelic frequency was estimated for each single-nucleotide polymorphism from genotyping data obtained for 154 animals from five horse breeds. Some of these frequencies varied significantly among different horse breeds. Domain architecture predictions for the three equine TLR protein sequences revealed several conserved regions within the variable leucine-rich repeats between the corresponding horse and cattle TLR proteins. A phylogenetic analysis did not indicate that any significant exchanges had occurred between paralogous TLR7 and TLR8 genes in 20 vertebrate species analyzed.

Original languageEnglish
Pages (from-to)529-539
Number of pages11
JournalImmunogenetics
Volume61
Issue number7
DOIs
StatePublished - Jul 2009

Bibliographical note

Funding Information:
Acknowledgments This work was supported by grant CI000216 from the National Center for Infectious Diseases, Centers for Disease Control and Prevention, to MAB and AAP.

Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.

Keywords

  • Equine TLR3
  • Equine TLR7
  • Equine TLR8
  • FISH
  • Phylogenetic tree
  • Single-nucleotide polymorphisms

ASJC Scopus subject areas

  • Immunology
  • Genetics

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