Characterization of the long-lasting activator protein-1 complex induced by kainic acid treatment

Zhehui Feng, Wanqin Zhang, Pearlie Hudson, Guoying Bing, Wei Feng, Jau Shyong Hong

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Kainic acid is known to induce seizures, neuronal damage and cell loss in the rat hippocampus. Our laboratory has shown that a single kainic acid injection elicits acute increases of activator protein-1 DNA-binding activity and this activity stays at an elevated level for 2 weeks after kainic acid injection. However, some pathological changes such as mossy fiber sprouting do not occur until 2-3 weeks after the kainic acid injection and the specific transcription factors regulating the long-term events after kainic acid treatment are not clear. To determine the involvement of activator protein-1 transcription factors in the long-term events after kainic acid treatment, gel mobility-shift and Western blot analyses were used. The results showed that two activator protein-1 complexes with different mobilities occur during the acute stage. However, only the faster- migrating complex as well as the 35-37-kDa fos-related antigen and Jan-D proteins were seen during the late stage. These results suggest that different activator protein-1 complexes exist at different stages after convulsions and that they regulate ensembles of different genes.

Original languageEnglish
Pages (from-to)53-59
Number of pages7
JournalBrain Research
Issue number1-2
StatePublished - Oct 3 1997


  • AP-1
  • Fra
  • Hippocampus
  • Jun-D
  • Kainic acid
  • Seizure
  • Transcription factor

ASJC Scopus subject areas

  • Neuroscience (all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


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