Characterization of the polyadenyllationm signal from the T-DNA-encoded octopine sysnthase gene

Margaret H. Macdonald, Bradley D. Mogen, Arthur G. Hunt

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

We have characterized the polyadenylation signal from the octopine synthase (ocs) gene. This signal directs mRNA 3′ end formation at a number of distinct sites.A combination of deletion and linker-substitution analyses revealed that each of these sites is controlled by multiple upstream sequence elements. Upstream sequences relatively far (>80 nt) from the ocs poly[A] sites were found to be needed for functioning of these sites. Upstream sequences nearer to poly[A] sites were also found to be involved in mRNA 3′ end formation in the ocs gene. In addition, a set of novel elements that mediates 3′ end choice was uncovered by deletion analysis of sequences downstream from the ocs polyadenylation sites. Our experiments indicate mRNA 3′ end formation in the ocs is controlled by a complex series of cis-acting signals, and suggest that the process of mRNA 3′ end formation might be linked to transcription termination.

Original languageEnglish
Pages (from-to)5575-5581
Number of pages7
JournalNucleic Acids Research
Volume19
Issue number20
DOIs
StatePublished - Oct 25 1991

Bibliographical note

Funding Information:
We thank Rebecca Richardson and Molly Goodenow for excellent technical assistance, and Brian Rymond for helpful suggestions. This work was supported by USDA Competitive Grants 85-CRCR-1-1810 and 89-37262-4835.

ASJC Scopus subject areas

  • Genetics

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