Chicken Muscle-Derived ACE2 Upregulating Peptide VVHPKESF Inhibits Angiotensin II-Stimulated Inflammation in Vascular Smooth Muscle Cells via the ACE2/Ang (1-7)/MasR Axis

Hongbing Fan, Wang Liao, Sandra T. Davidge, Jianping Wu

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

This study aimed to evaluate the modulatory effects of four chicken muscle-derived peptides [VRP, LKY, VRY, and VVHPKESF (V-F)] on angiotensin II (Ang II)-induced inflammation in rat vascular smooth muscle A7r5 cells. Only V-F could significantly attenuate Ang II-stimulated inflammation via the inhibition of NF-κB and p38 MAPK signaling, being dependent on the Mas receptor (MasR) not on the Ang II type 1 or type 2 receptor (AT1R or AT2R). V-F accelerated Ang II degradation by enhancing cellular ACE2 activity, which was due to ACE2 upregulation other than a direct ACE2 activation. These findings demonstrated that V-F ameliorated Ang II-induced inflammation in A7r5 cells via the ACE2/Ang (1-7)/MasR axis. Three peptide metabolites of V-F─VHPKESF, PKESF, and SF─were identified but were not considered major contributors to V-F's bioactivity. The regulation of peptide V-F on vascular inflammation supported its functional food or nutraceutical application in the prevention and treatment of hypertension and cardiovascular diseases.

Original languageEnglish
Pages (from-to)6397-6406
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume70
Issue number21
DOIs
StatePublished - Jun 1 2022

Bibliographical note

Publisher Copyright:
© 2022 American Chemical Society. All rights reserved.

Keywords

  • ACE
  • ACE2
  • bioactive peptides
  • chicken
  • inflammation
  • metabolism
  • signaling pathways
  • vascular smooth muscle cells

ASJC Scopus subject areas

  • General Chemistry
  • General Agricultural and Biological Sciences

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