Abstract
Scope: This study aims to investigate the protective effects of four chicken muscle-derived peptides Val-Arg-Pro (VRP), Leu-Lys-Tyr (LKY), Val-Arg-Tyr (VRY), and Val-Val-His-Pro-Lys-Glu-Ser-Phe [VVHPKESF (V-F)] on tumor necrosis factor alpha (TNFα)-induced endothelial inflammation and oxidative stress in human vascular endothelial EA.hy926 cells. Methods and results: Inflammation and oxidative stress are induced in EA.hy926 cells by TNFα (10 ng mL−1) treatment for different periods of time. Inflammatory proteins and signaling molecules including inducible nitric oxide synthase, intracellular cell adhesion molecule-1, vascular cell adhesion molecule-1 (VCAM-1), cyclooxygenase 2 (COX2), nuclear factor kappa B (NF-κB), mitogen-activated protein kinases (MAPKs), and TNFα receptor 1 (TNFR1) are measured by qRT-PCR or western blotting; soluble TNFR1 level and nicotinamide adenine dinucleotide phosphate NADPH) oxidase activity are determined by Elisa kits; superoxide is measured by dihydroethidium staining. Only V-F treatment inhibits the expression of VCAM-1 and COX2, via suppressing NF-κB and p38 MAPK signaling, respectively, while reduced oxidative stress via the inhibition of NADPH oxidase activity; V-F treatment attenuates both gene and protein expressions of TNFR1. Conclusion: V-F treatment ameliorates TNFα-induced endothelial inflammation and oxidative stress likely via the inhibition of TNFR1 signaling, suggesting its potential as a functional food ingredient or nutraceutical in the prevention and treatment of hypertension and cardiovascular diseases.
Original language | English |
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Article number | 2200184 |
Journal | Molecular Nutrition and Food Research |
Volume | 66 |
Issue number | 17 |
DOIs | |
State | Published - Sep 2022 |
Bibliographical note
Funding Information:This work was supported by the Natural Sciences and Engineering Research Council of Canada and Egg Farmers of Canada. The authors would also like to acknowledge the financial support received from Alberta Innovates Technology Futures, the Killam Trusts, and the American Oil Chemists’ Society (AOCS) Thomas H. Smouse Memorial Fellowship. Special thanks to Biorender.com for creating the graphical abstract.
Publisher Copyright:
© 2022 Wiley-VCH GmbH.
Keywords
- bioactive peptides
- cellular mechanism
- endothelial cell
- inflammation
- meat
- NADPH
- oxidative stress
- TNFR1
ASJC Scopus subject areas
- Biotechnology
- Food Science