We have identified and characterized an ortholog of the putative mammalian clock gene cryptochrome 2 (Cry2) in the chicken, Gallus domesticus. Northern blot analysis of gCry2 mRNA indicates widespread distribution in central nervous and peripheral tissues, with very high expression in pineal and retina. In situ hybridization of chick brain and retina reveals expression in photoreceptors and in visual and circadian system structures. Expression is rhythmic; mRNA levels predominate in late subjective night. The present data suggests that gCry2 is a candidate avian clock gene and/or photopigment and set the stage for functional studies of gCry2.
|Number of pages||6|
|State||Published - Feb 27 2002|
Bibliographical noteFunding Information:
This work was partly supported by NIH Grant RO1 NS35822 and PO1 NS39546 to V.M.C. Research in the laboratory of N.W.C. was supported in part by the Royal Society and the Wellcome Trust (Travel Research Award). We thank Dr. Deborah Bell-Pedersen, Dr. Thomas McKnight, Dr. David Earnest, and Dr. Terry Thomas for guidance during the course of these studies and Mrs. Barbara Earnest for animal care support.
- Circadian rhythm
- Clock gene
- Pineal gland
ASJC Scopus subject areas
- Structural Biology
- Molecular Biology
- Cell Biology