Chickens' Cry2: Molecular analysis of an avian cryptochrome in retinal and pineal photoreceptors

Michael J. Bailey; Nelson W. Chong; Jin Xiong; Vincent M. Cassone

doi:10.1016/S0014-5793(02)02276-7

Chickens' Cry2: Molecular analysis of an avian cryptochrome in retinal and pineal photoreceptors

Michael J. Bailey, Nelson W. Chong, Jin Xiong, Vincent M. Cassone

Research output: Contribution to journal › Article › peer-review

96 Scopus citations

Abstract

We have identified and characterized an ortholog of the putative mammalian clock gene cryptochrome 2 (Cry2) in the chicken, Gallus domesticus. Northern blot analysis of gCry2 mRNA indicates widespread distribution in central nervous and peripheral tissues, with very high expression in pineal and retina. In situ hybridization of chick brain and retina reveals expression in photoreceptors and in visual and circadian system structures. Expression is rhythmic; mRNA levels predominate in late subjective night. The present data suggests that gCry2 is a candidate avian clock gene and/or photopigment and set the stage for functional studies of gCry2.

Original language	English
Pages (from-to)	169-174
Number of pages	6
Journal	FEBS Letters
Volume	513
Issue number	2-3
DOIs	https://doi.org/10.1016/S0014-5793(02)02276-7
State	Published - Feb 27 2002

Bibliographical note

Funding Information:
This work was partly supported by NIH Grant RO1 NS35822 and PO1 NS39546 to V.M.C. Research in the laboratory of N.W.C. was supported in part by the Royal Society and the Wellcome Trust (Travel Research Award). We thank Dr. Deborah Bell-Pedersen, Dr. Thomas McKnight, Dr. David Earnest, and Dr. Terry Thomas for guidance during the course of these studies and Mrs. Barbara Earnest for animal care support.

Funding

This work was partly supported by NIH Grant RO1 NS35822 and PO1 NS39546 to V.M.C. Research in the laboratory of N.W.C. was supported in part by the Royal Society and the Wellcome Trust (Travel Research Award). We thank Dr. Deborah Bell-Pedersen, Dr. Thomas McKnight, Dr. David Earnest, and Dr. Terry Thomas for guidance during the course of these studies and Mrs. Barbara Earnest for animal care support.

Funders	Funder number
National Institutes of Health (NIH)	RO1 NS35822
National Institute of Neurological Disorders and Stroke	P01NS039546
Wellcome Trust
Royal Society of Medicine

Keywords

Chicken
Circadian rhythm
Clock gene
Cryptochrome
Pineal gland
Retina

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(02)02276-7

Cite this

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title = "Chickens' Cry2: Molecular analysis of an avian cryptochrome in retinal and pineal photoreceptors",

abstract = "We have identified and characterized an ortholog of the putative mammalian clock gene cryptochrome 2 (Cry2) in the chicken, Gallus domesticus. Northern blot analysis of gCry2 mRNA indicates widespread distribution in central nervous and peripheral tissues, with very high expression in pineal and retina. In situ hybridization of chick brain and retina reveals expression in photoreceptors and in visual and circadian system structures. Expression is rhythmic; mRNA levels predominate in late subjective night. The present data suggests that gCry2 is a candidate avian clock gene and/or photopigment and set the stage for functional studies of gCry2.",

keywords = "Chicken, Circadian rhythm, Clock gene, Cryptochrome, Pineal gland, Retina",

author = "Bailey, {Michael J.} and Chong, {Nelson W.} and Jin Xiong and Cassone, {Vincent M.}",

note = "Funding Information: This work was partly supported by NIH Grant RO1 NS35822 and PO1 NS39546 to V.M.C. Research in the laboratory of N.W.C. was supported in part by the Royal Society and the Wellcome Trust (Travel Research Award). We thank Dr. Deborah Bell-Pedersen, Dr. Thomas McKnight, Dr. David Earnest, and Dr. Terry Thomas for guidance during the course of these studies and Mrs. Barbara Earnest for animal care support.",

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AU - Cassone, Vincent M.

N1 - Funding Information: This work was partly supported by NIH Grant RO1 NS35822 and PO1 NS39546 to V.M.C. Research in the laboratory of N.W.C. was supported in part by the Royal Society and the Wellcome Trust (Travel Research Award). We thank Dr. Deborah Bell-Pedersen, Dr. Thomas McKnight, Dr. David Earnest, and Dr. Terry Thomas for guidance during the course of these studies and Mrs. Barbara Earnest for animal care support.

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AB - We have identified and characterized an ortholog of the putative mammalian clock gene cryptochrome 2 (Cry2) in the chicken, Gallus domesticus. Northern blot analysis of gCry2 mRNA indicates widespread distribution in central nervous and peripheral tissues, with very high expression in pineal and retina. In situ hybridization of chick brain and retina reveals expression in photoreceptors and in visual and circadian system structures. Expression is rhythmic; mRNA levels predominate in late subjective night. The present data suggests that gCry2 is a candidate avian clock gene and/or photopigment and set the stage for functional studies of gCry2.

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KW - Circadian rhythm

KW - Clock gene

KW - Cryptochrome

KW - Pineal gland

KW - Retina

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Chickens' Cry2: Molecular analysis of an avian cryptochrome in retinal and pineal photoreceptors

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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