Abstract
In spite of devastating impact of mosquito borne pathogens on humans, widespread resistance to chemical insecticides and environmental concerns from residual toxicity limit mosquito control strategies. We tested three nanoparticles, chitosan, carbon quantum dot (CQD), and silica complexed with dsRNA, to target two mosquito genes (SNF7 and SRC) for controlling Aedes aegypti larvae. Relative mRNA levels were quantified using qRT-PCR to evaluate knockdown efficiency in nanoparticle-dsRNA treated larvae. The knockdown efficiency of target genes correlated with dsRNA mediated larval mortality. Among the three nanoparticles tested, CQD was the most efficient carrier for dsRNA retention, delivery, and thereby causing gene silencing and mortality in Ae. aegypti.
Original language | English |
---|---|
Pages (from-to) | 19530-19535 |
Number of pages | 6 |
Journal | ACS Applied Materials and Interfaces |
Volume | 7 |
Issue number | 35 |
DOIs | |
State | Published - Sep 9 2015 |
Bibliographical note
Publisher Copyright:© 2015 American Chemical Society.
Keywords
- CQD
- Chitosan
- RNAi
- SNF7
- SRC
- dsRNA
- mosquito
ASJC Scopus subject areas
- Materials Science (all)