Chitosan, Carbon Quantum Dot, and Silica Nanoparticle Mediated dsRNA Delivery for Gene Silencing in Aedes aegypti: A Comparative Analysis

Sumistha Das; Nitai Debnath; Yingjun Cui; Jason Unrine; Subba Reddy Palli

doi:10.1021/acsami.5b05232

Chitosan, Carbon Quantum Dot, and Silica Nanoparticle Mediated dsRNA Delivery for Gene Silencing in Aedes aegypti: A Comparative Analysis

Sumistha Das, Nitai Debnath, Yingjun Cui, Jason Unrine, Subba Reddy Palli

Research output: Contribution to journal › Article › peer-review

108 Scopus citations

Abstract

In spite of devastating impact of mosquito borne pathogens on humans, widespread resistance to chemical insecticides and environmental concerns from residual toxicity limit mosquito control strategies. We tested three nanoparticles, chitosan, carbon quantum dot (CQD), and silica complexed with dsRNA, to target two mosquito genes (SNF7 and SRC) for controlling Aedes aegypti larvae. Relative mRNA levels were quantified using qRT-PCR to evaluate knockdown efficiency in nanoparticle-dsRNA treated larvae. The knockdown efficiency of target genes correlated with dsRNA mediated larval mortality. Among the three nanoparticles tested, CQD was the most efficient carrier for dsRNA retention, delivery, and thereby causing gene silencing and mortality in Ae. aegypti.

Original language	English
Pages (from-to)	19530-19535
Number of pages	6
Journal	ACS Applied Materials and Interfaces
Volume	7
Issue number	35
DOIs	https://doi.org/10.1021/acsami.5b05232
State	Published - Sep 9 2015

Bibliographical note

Publisher Copyright:
© 2015 American Chemical Society.

Keywords

CQD
Chitosan
RNAi
SNF7
SRC
dsRNA
mosquito

ASJC Scopus subject areas

Materials Science (all)

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10.1021/acsami.5b05232

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abstract = "In spite of devastating impact of mosquito borne pathogens on humans, widespread resistance to chemical insecticides and environmental concerns from residual toxicity limit mosquito control strategies. We tested three nanoparticles, chitosan, carbon quantum dot (CQD), and silica complexed with dsRNA, to target two mosquito genes (SNF7 and SRC) for controlling Aedes aegypti larvae. Relative mRNA levels were quantified using qRT-PCR to evaluate knockdown efficiency in nanoparticle-dsRNA treated larvae. The knockdown efficiency of target genes correlated with dsRNA mediated larval mortality. Among the three nanoparticles tested, CQD was the most efficient carrier for dsRNA retention, delivery, and thereby causing gene silencing and mortality in Ae. aegypti.",

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AU - Das, Sumistha

AU - Debnath, Nitai

AU - Cui, Yingjun

AU - Unrine, Jason

AU - Palli, Subba Reddy

PY - 2015/9/9

Y1 - 2015/9/9

N2 - In spite of devastating impact of mosquito borne pathogens on humans, widespread resistance to chemical insecticides and environmental concerns from residual toxicity limit mosquito control strategies. We tested three nanoparticles, chitosan, carbon quantum dot (CQD), and silica complexed with dsRNA, to target two mosquito genes (SNF7 and SRC) for controlling Aedes aegypti larvae. Relative mRNA levels were quantified using qRT-PCR to evaluate knockdown efficiency in nanoparticle-dsRNA treated larvae. The knockdown efficiency of target genes correlated with dsRNA mediated larval mortality. Among the three nanoparticles tested, CQD was the most efficient carrier for dsRNA retention, delivery, and thereby causing gene silencing and mortality in Ae. aegypti.

AB - In spite of devastating impact of mosquito borne pathogens on humans, widespread resistance to chemical insecticides and environmental concerns from residual toxicity limit mosquito control strategies. We tested three nanoparticles, chitosan, carbon quantum dot (CQD), and silica complexed with dsRNA, to target two mosquito genes (SNF7 and SRC) for controlling Aedes aegypti larvae. Relative mRNA levels were quantified using qRT-PCR to evaluate knockdown efficiency in nanoparticle-dsRNA treated larvae. The knockdown efficiency of target genes correlated with dsRNA mediated larval mortality. Among the three nanoparticles tested, CQD was the most efficient carrier for dsRNA retention, delivery, and thereby causing gene silencing and mortality in Ae. aegypti.

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Chitosan, Carbon Quantum Dot, and Silica Nanoparticle Mediated dsRNA Delivery for Gene Silencing in Aedes aegypti: A Comparative Analysis

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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