Chitosan, Carbon Quantum Dot, and Silica Nanoparticle Mediated dsRNA Delivery for Gene Silencing in Aedes aegypti: A Comparative Analysis

Sumistha Das, Nitai Debnath, Yingjun Cui, Jason Unrine, Subba Reddy Palli

Research output: Contribution to journalArticlepeer-review

139 Scopus citations

Abstract

In spite of devastating impact of mosquito borne pathogens on humans, widespread resistance to chemical insecticides and environmental concerns from residual toxicity limit mosquito control strategies. We tested three nanoparticles, chitosan, carbon quantum dot (CQD), and silica complexed with dsRNA, to target two mosquito genes (SNF7 and SRC) for controlling Aedes aegypti larvae. Relative mRNA levels were quantified using qRT-PCR to evaluate knockdown efficiency in nanoparticle-dsRNA treated larvae. The knockdown efficiency of target genes correlated with dsRNA mediated larval mortality. Among the three nanoparticles tested, CQD was the most efficient carrier for dsRNA retention, delivery, and thereby causing gene silencing and mortality in Ae. aegypti.

Original languageEnglish
Pages (from-to)19530-19535
Number of pages6
JournalACS Applied Materials and Interfaces
Volume7
Issue number35
DOIs
StatePublished - Sep 9 2015

Bibliographical note

Publisher Copyright:
© 2015 American Chemical Society.

Keywords

  • CQD
  • Chitosan
  • RNAi
  • SNF7
  • SRC
  • dsRNA
  • mosquito

ASJC Scopus subject areas

  • Materials Science (all)

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