TY - JOUR
T1 - Chitosan, Carbon Quantum Dot, and Silica Nanoparticle Mediated dsRNA Delivery for Gene Silencing in Aedes aegypti
T2 - A Comparative Analysis
AU - Das, Sumistha
AU - Debnath, Nitai
AU - Cui, Yingjun
AU - Unrine, Jason
AU - Palli, Subba Reddy
N1 - Publisher Copyright:
© 2015 American Chemical Society.
PY - 2015/9/9
Y1 - 2015/9/9
N2 - In spite of devastating impact of mosquito borne pathogens on humans, widespread resistance to chemical insecticides and environmental concerns from residual toxicity limit mosquito control strategies. We tested three nanoparticles, chitosan, carbon quantum dot (CQD), and silica complexed with dsRNA, to target two mosquito genes (SNF7 and SRC) for controlling Aedes aegypti larvae. Relative mRNA levels were quantified using qRT-PCR to evaluate knockdown efficiency in nanoparticle-dsRNA treated larvae. The knockdown efficiency of target genes correlated with dsRNA mediated larval mortality. Among the three nanoparticles tested, CQD was the most efficient carrier for dsRNA retention, delivery, and thereby causing gene silencing and mortality in Ae. aegypti.
AB - In spite of devastating impact of mosquito borne pathogens on humans, widespread resistance to chemical insecticides and environmental concerns from residual toxicity limit mosquito control strategies. We tested three nanoparticles, chitosan, carbon quantum dot (CQD), and silica complexed with dsRNA, to target two mosquito genes (SNF7 and SRC) for controlling Aedes aegypti larvae. Relative mRNA levels were quantified using qRT-PCR to evaluate knockdown efficiency in nanoparticle-dsRNA treated larvae. The knockdown efficiency of target genes correlated with dsRNA mediated larval mortality. Among the three nanoparticles tested, CQD was the most efficient carrier for dsRNA retention, delivery, and thereby causing gene silencing and mortality in Ae. aegypti.
KW - CQD
KW - Chitosan
KW - RNAi
KW - SNF7
KW - SRC
KW - dsRNA
KW - mosquito
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U2 - 10.1021/acsami.5b05232
DO - 10.1021/acsami.5b05232
M3 - Article
C2 - 26291176
AN - SCOPUS:84941071832
SN - 1944-8244
VL - 7
SP - 19530
EP - 19535
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 35
ER -