TY - JOUR
T1 - Chitosan-Collagen Biomembrane Embedded With Calcium-Aluminate Enhances Dentinogenic Potential Of Pulp Cells
AU - Soares, Diana Gabriela
AU - Rosseto, Hebert Luis
AU - Basso, Fernanda Goncalves
AU - Scheffel, Debora Salles
AU - Hebling, Josimeri
AU - Costa, Carlos Alberto De Souza
N1 - Publisher Copyright:
© 2016,Brazilian Oral Research.All Rights Reserved
PY - 2016
Y1 - 2016
N2 - The Development Of Biomaterials Capable Of Driving Dental Pulp Stem Cell Differentiation Into Odontoblast-Like Cells Able To Secrete Reparative Dentin Is The Goal Of Current Conservative Dentistry. In The Present Investigation, A Biomembrane (Bm) Composed Of A Chitosan/Collagen Matrix Embedded With Calcium-Aluminate Microparticles Was Tested. The Bm Was Produced By Mixing Collagen Gel With A Chitosan Solution (2:1), And Then Adding Bioactive Calcium-Aluminate Cement As The Mineral Phase. An Inert Material (Polystyrene) Was Used As The Negative Control. Human Dental Pulp Cells Were Seeded Onto The Surface Of Certain Materials, And The Cytocompatibility Was Evaluated By Cell Proliferation And Cell Morphology, Assessed After 1, 7, 14 And 28 Days In Culture. The Odontoblastic Differentiation Was Evaluated By Measuring Alkaline Phosphatase (Alp) Activity, Total Protein Production, Gene Expression Of Dmp-1/Dspp And Mineralized Nodule Deposition. The Pulp Cells Were Able To Attach Onto The Bm Surface And Spread, Displaying A Faster Proliferative Rate At Initial Periods Than That Of The Control Cells. The Bm Also Acted On The Cells To Induce More Intense Alp Activity, Protein Production At 14 Days, And Higher Gene Expression Of Dspp And Dmp-1 At 28 Days, Leading To The Deposition Of About Five Times More Mineralized Matrix Than The Cells In The Control Group. Therefore, The Experimental Biomembrane Induced The Differentiation Of Pulp Cells Into Odontoblast-Like Cells Featuring A Highly Secretory Phenotype.
AB - The Development Of Biomaterials Capable Of Driving Dental Pulp Stem Cell Differentiation Into Odontoblast-Like Cells Able To Secrete Reparative Dentin Is The Goal Of Current Conservative Dentistry. In The Present Investigation, A Biomembrane (Bm) Composed Of A Chitosan/Collagen Matrix Embedded With Calcium-Aluminate Microparticles Was Tested. The Bm Was Produced By Mixing Collagen Gel With A Chitosan Solution (2:1), And Then Adding Bioactive Calcium-Aluminate Cement As The Mineral Phase. An Inert Material (Polystyrene) Was Used As The Negative Control. Human Dental Pulp Cells Were Seeded Onto The Surface Of Certain Materials, And The Cytocompatibility Was Evaluated By Cell Proliferation And Cell Morphology, Assessed After 1, 7, 14 And 28 Days In Culture. The Odontoblastic Differentiation Was Evaluated By Measuring Alkaline Phosphatase (Alp) Activity, Total Protein Production, Gene Expression Of Dmp-1/Dspp And Mineralized Nodule Deposition. The Pulp Cells Were Able To Attach Onto The Bm Surface And Spread, Displaying A Faster Proliferative Rate At Initial Periods Than That Of The Control Cells. The Bm Also Acted On The Cells To Induce More Intense Alp Activity, Protein Production At 14 Days, And Higher Gene Expression Of Dspp And Dmp-1 At 28 Days, Leading To The Deposition Of About Five Times More Mineralized Matrix Than The Cells In The Control Group. Therefore, The Experimental Biomembrane Induced The Differentiation Of Pulp Cells Into Odontoblast-Like Cells Featuring A Highly Secretory Phenotype.
KW - Biocompatible Materials
KW - Dental Pulp
KW - Dentin
KW - Stem Cells
KW - Tissue Engineering
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U2 - 10.1590/1807-3107BOR-2016.VOL30.0054
DO - 10.1590/1807-3107BOR-2016.VOL30.0054
M3 - Article
C2 - 27119587
AN - SCOPUS:84991062414
SN - 1806-8324
VL - 30
SP - 1
EP - 10
JO - Brazilian Oral Research
JF - Brazilian Oral Research
IS - 1
ER -