Several general anesthetics reduce dopamine (DA) neuronal activity and release. However, anesthetic-induced alterations in DA transporter (DAT) function are unclear. We used high-speed chronoamperometry to examine how urethane, chloral hydrate and ethanol affected clearance of locally-applied DA in the dorsal striatum of unrestrained rats. Maximal DA signal amplitudes (Amax) and signal decay rate constants (k) were monitored continuously from the awake to anesthetized state. Urethane did not significantly change Amax or k. Chloral hydrate and ethanol potentiated Amax by 32% and 37%, respectively, over baseline, indicative of DAT inhibition. These effects were not additive. Only ethanol increased k, an effect blocked by chloral hydrate. These results suggest differential effects of anesthetics on striatal DAT function, with chloral hydrate and ethanol significantly altering DAT activity but urethane producing only minimal effects.
|Number of pages||4|
|State||Published - May 29 2003|
Bibliographical noteFunding Information:
This work was supported by National Institutes of Health grants DA04216 (NRZ), AG06434 and NS39787 (GAG), Training Grant GM07635 (JS) and Research Scientist Awards DA15050 (NRZ) and MH01245 (GAG).
- Dopamine transporter
- General anesthetics
ASJC Scopus subject areas
- Neuroscience (all)