Chlorogenic acid methyl ester exerts strong anti-inflammatory effects: Via inhibiting the COX-2/NLRP3/NF-κB pathway

Lang Zhang, Ya Fan, Hanwen Su, Li Wu, Yuying Huang, Lei Zhao, Bing Han, Guangwen Shu, Meixian Xiang, Jin Ming Yang

Research output: Contribution to journalArticlepeer-review

37 Scopus citations


The objective of this study was to investigate the anti-inflammatory effect of chlorogenic acid methyl ester (CME) and the molecular mechanism involved, through using non-infectious inflammation and infectious inflammation animal models as well as lipopolysaccharide (LPS)-stimulated mouse macrophage RAW264.7 cell models. Our results demonstrated that CME markedly inhibited ear swelling, paw swelling and granuloma swelling, and decreased intraperitoneal capillary permeability in non-infectious inflammation. Moreover, CME significantly alleviated the pathological damage of the lung tissue, reduced the levels of PGE2 and IL-1β in the serum and the protein expression levels of related-inflammatory factors in the lung tissue of LPS-induced mice with acute lung injury (ALI). In addition, CME affected the RAW264.7 cell cycle and inhibited the protein expressions of COX-2 and NLRP3 and prevented the phosphorylation of NF-κB p65 in RAW264.7 cells treated with LPS. These observations not only validated the anti-inflammatory effects of CME, but also revealed the underlying molecular basis, which involves the down-regulation of the expression of inflammatory factors and blockade of the COX-2/NLRP3/NF-κB signaling pathway.

Original languageEnglish
Pages (from-to)6155-6164
Number of pages10
JournalFood and Function
Issue number12
StatePublished - 2018

Bibliographical note

Funding Information:
This work was supported by the Natural Science Foundation of China (31200264), the Natural Science Foundation of Hubei Province (2015BCA268 and 2016CFB648) and the Natural Science Foundation of Wuhan City (2016070204020162).

Publisher Copyright:
© 2018 The Royal Society of Chemistry.

ASJC Scopus subject areas

  • Food Science


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