Chloroplast genome organisation in sugar beet and maize

Timothy Brears; Christopher L. Schardl; David M. Lonsdale

doi:10.1007/BF00021486

Chloroplast genome organisation in sugar beet and maize

Timothy Brears, Christopher L. Schardl, David M. Lonsdale

Plant Pathology

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The XhoI and SmaI restriction map of the chloroplast genome from the fertile cytoplasm of sugar beet has been constructed from overlapping cosmid clones. The genome was found to be typical of that of a dicotyledonous species, being 147.3 kb in size and having an inverted repeat. RbcL for the large subunit of ribulose-1,5-bisphosphate carboxylase, psbA for the 32 kD protein of the photosystem II reaction centre, and the 16S ribosomal RNA were located using heterologous probes. In both sugar beet and maize the inverted repeats recombine giving two isomeric forms of the genome.

Original language	English
Pages (from-to)	171-177
Number of pages	7
Journal	Plant Molecular Biology
Volume	6
Issue number	3
DOIs	https://doi.org/10.1007/BF00021486
State	Published - May 1986

Keywords

chloroplast DNA
cosmid cloning
maize
recombination
sugar beet

ASJC Scopus subject areas

Agronomy and Crop Science
Genetics
Plant Science

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10.1007/BF00021486

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title = "Chloroplast genome organisation in sugar beet and maize",

abstract = "The XhoI and SmaI restriction map of the chloroplast genome from the fertile cytoplasm of sugar beet has been constructed from overlapping cosmid clones. The genome was found to be typical of that of a dicotyledonous species, being 147.3 kb in size and having an inverted repeat. RbcL for the large subunit of ribulose-1,5-bisphosphate carboxylase, psbA for the 32 kD protein of the photosystem II reaction centre, and the 16S ribosomal RNA were located using heterologous probes. In both sugar beet and maize the inverted repeats recombine giving two isomeric forms of the genome.",

keywords = "chloroplast DNA, cosmid cloning, maize, recombination, sugar beet",

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T1 - Chloroplast genome organisation in sugar beet and maize

AU - Brears, Timothy

AU - Schardl, Christopher L.

AU - Lonsdale, David M.

PY - 1986/5

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N2 - The XhoI and SmaI restriction map of the chloroplast genome from the fertile cytoplasm of sugar beet has been constructed from overlapping cosmid clones. The genome was found to be typical of that of a dicotyledonous species, being 147.3 kb in size and having an inverted repeat. RbcL for the large subunit of ribulose-1,5-bisphosphate carboxylase, psbA for the 32 kD protein of the photosystem II reaction centre, and the 16S ribosomal RNA were located using heterologous probes. In both sugar beet and maize the inverted repeats recombine giving two isomeric forms of the genome.

AB - The XhoI and SmaI restriction map of the chloroplast genome from the fertile cytoplasm of sugar beet has been constructed from overlapping cosmid clones. The genome was found to be typical of that of a dicotyledonous species, being 147.3 kb in size and having an inverted repeat. RbcL for the large subunit of ribulose-1,5-bisphosphate carboxylase, psbA for the 32 kD protein of the photosystem II reaction centre, and the 16S ribosomal RNA were located using heterologous probes. In both sugar beet and maize the inverted repeats recombine giving two isomeric forms of the genome.

KW - chloroplast DNA

KW - cosmid cloning

KW - maize

KW - recombination

KW - sugar beet

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JF - Plant Molecular Biology

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Chloroplast genome organisation in sugar beet and maize

Abstract

Keywords

ASJC Scopus subject areas

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