Chromatin isolation by RNA purification (ChIRP) and its applications

Changhai Tian, Guoku Hu

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Chromatin isolation by RNA purification (ChIRP) is a valuable method to map the genomic binding profile of chromatin-associated RNAs, such as long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs). ChIRP was initially designed by Chu and colleagues to investigate RNA bound DNA using affinity-probes to a target RNA, and its application has been recently extended to also identify RNAs and proteins that interact with the target RNA. Since its original development in 2011, ChIRP has been increasingly used in various fields of biological research which has, in turn, resulted in an enhanced understanding of the dynamics of RNA-chromatin interactions and their roles in health and diseases. This chapter describes the methodology and applications of ChIRP.

Original languageEnglish
Title of host publicationEpigenetics Methods
Pages507-521
Number of pages15
ISBN (Electronic)9780128194140
DOIs
StatePublished - Jan 1 2020

Bibliographical note

Publisher Copyright:
© 2020 Elsevier Inc. All rights reserved.

Keywords

  • ChIRP
  • Chromatin
  • Genome-wide binding
  • LncRNA
  • RNA-chromatin interaction

ASJC Scopus subject areas

  • Medicine (all)
  • Biochemistry, Genetics and Molecular Biology (all)

Fingerprint

Dive into the research topics of 'Chromatin isolation by RNA purification (ChIRP) and its applications'. Together they form a unique fingerprint.

Cite this