Chromosomal-level genome assembly of potato tuberworm, Phthorimaea operculella: a pest of solanaceous crops

Mengdi Zhang; Xinyue Cheng; Runmao Lin; Bingyan Xie; Ralf Nauen; Silvia I. Rondon; Jorge A. Zavala; Subba Reddy Palli; Suhua Li; Xingyao Xiong; Wenwu Zhou; Yulin Gao

doi:10.1038/s41597-022-01859-5

Chromosomal-level genome assembly of potato tuberworm, Phthorimaea operculella: a pest of solanaceous crops

Mengdi Zhang, Xinyue Cheng, Runmao Lin, Bingyan Xie, Ralf Nauen, Silvia I. Rondon, Jorge A. Zavala, Subba Reddy Palli, Suhua Li, Xingyao Xiong, Wenwu Zhou, Yulin Gao

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Abstract

The potato tuberworm, Phthorimaea operculella Zeller, is an oligophagous pest feeding on crops mainly belonging to the family Solanaceae. It is one of the most destructive pests of potato worldwide and attacks foliage and tubers in the field and in storage. However, the lack of a high-quality reference genome has hindered the association of phenotypic traits with their genetic basis. Here, we report on the genome assembly of P. operculella at the chromosomal level. Using Illumina, Nanopore and Hi-C sequencing, a 648.2 Mb genome was generated from 665 contigs, with an N50 length of 3.2 Mb, and 92.0% (596/648.2 Mb) of the assembly was anchored to 29 chromosomes. In total, 16619 genes were annotated, and 92.4% of BUSCO genes were fully represented. The chromosome-level genome of P. operculella will provide a significant resource for understanding the genetic basis for the biological study of this insect, and for promoting the integrative management of this pest in future.

Original language	English
Article number	748
Journal	Scientific data
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41597-022-01859-5
State	Published - Dec 2022

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© 2022, The Author(s).

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Information Systems
Education
Computer Science Applications
Statistics, Probability and Uncertainty
Library and Information Sciences

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title = "Chromosomal-level genome assembly of potato tuberworm, Phthorimaea operculella: a pest of solanaceous crops",

abstract = "The potato tuberworm, Phthorimaea operculella Zeller, is an oligophagous pest feeding on crops mainly belonging to the family Solanaceae. It is one of the most destructive pests of potato worldwide and attacks foliage and tubers in the field and in storage. However, the lack of a high-quality reference genome has hindered the association of phenotypic traits with their genetic basis. Here, we report on the genome assembly of P. operculella at the chromosomal level. Using Illumina, Nanopore and Hi-C sequencing, a 648.2 Mb genome was generated from 665 contigs, with an N50 length of 3.2 Mb, and 92.0% (596/648.2 Mb) of the assembly was anchored to 29 chromosomes. In total, 16619 genes were annotated, and 92.4% of BUSCO genes were fully represented. The chromosome-level genome of P. operculella will provide a significant resource for understanding the genetic basis for the biological study of this insect, and for promoting the integrative management of this pest in future.",

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AU - Xie, Bingyan

AU - Nauen, Ralf

AU - Rondon, Silvia I.

AU - Zavala, Jorge A.

AU - Palli, Subba Reddy

AU - Li, Suhua

AU - Xiong, Xingyao

AU - Zhou, Wenwu

AU - Gao, Yulin

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Chromosomal-level genome assembly of potato tuberworm, Phthorimaea operculella: a pest of solanaceous crops

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