Chromosome 16 allelic loss analysis of a large set of microdissected prostate carcinomas

Stephen E. Strup, Rudy O. Pozzatti, Charles D. Florence, Michael R. Emmert-Buck, Paul H. Duray, Lance A. Liotta, David G. Bostwick, W. Marston Linehan, Cathy D. Vocke

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Purpose: To perform loss of heterozygosity (LOH) analysis on chromosome 16 in 102 highly purified DNA samples isolated from one or more adenocarcinomas, prostatic intraepithelial neoplasia (PIN), and matched benign prostatic epithelium from 95 radical prostatectomy patients. Materials and Methods: Specimens were procured by microdissection of frozen tissue samples, thus ensuring that highly select pure populations of cells were obtained for DNA extraction and LOH analysis. Multiple microsatellite markers were used to determine allelic loss on chromosome 16q. Results: Overall loss on 16q was seen in 31% of the cancers, and occurred more frequently in high stage cancers than low stage cancers. In contrast, allelic loss in PIN failed to exceed 6% at any of the loci that were examined. Conclusions: These results suggest that inactivation of a putative tumor suppressor gene on 16q may be involved in the progression of some prostate cancers.

Original languageEnglish
Pages (from-to)590-594
Number of pages5
JournalJournal of Urology
Issue number2
StatePublished - Aug 1999


  • Loss of heterozygosity
  • Microsatellite polymorphism
  • Prostate carcinoma
  • Tissue microdissection
  • Tumor suppressor gene

ASJC Scopus subject areas

  • Urology


Dive into the research topics of 'Chromosome 16 allelic loss analysis of a large set of microdissected prostate carcinomas'. Together they form a unique fingerprint.

Cite this