Chronic intermittent L-DOPA treatment induces changes in dopamine release

Martin Lundblad, Sara Af Bjerkén, M. Angela Cenci, Francois Pomerleau, Greg A. Gerhardt, Ingrid Strömberg

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


3,4-Dihydroxyphenyl-L-alanine (L-DOPA)-induced dyskinesia often develops as a side effect of chronic L-DOPA therapy. This study was undertaken to investigate dopamine (DA) release upon L-DOPA treatment. Chronoamperometric measurements were performed in unilaterally DA-depleted rats, chronically treated with L-DOPA, resulting in dyskinetic and non-dyskinetic animals. Normal and lesioned L-DOPA naïve animals were used as controls. Potassium-evoked DA releases were significantly reduced in intact sides of animals undertaken chronic L-DOPA treatment, independent on dyskinetic behavior. Acute L-DOPA further attenuated the amplitude of the DA release in the control sides. In DA-depleted striata, no difference was found in potassium-evoked DA releases, and acute L-DOPA did not affect the amplitude. While immunoreactivity to serotonin uptake transporter was higher in lesioned striata of animals displaying dyskinetic behavior, no correlation could be documented between serotonin transporter-positive nerve fiber density and the amplitude of released DA. In conclusions, the amplitude of potassium-evoked DA release is attenuated in intact striatum after chronic intermittent L-DOPA treatment. No change in amplitude was found in DA-denervated sides of either dyskinetic or non-dyskinetic animals, while release kinetics were changed. This indicates the importance of studying DA release dynamics for the understanding of both beneficial and adverse effects of L-DOPA replacement therapy.

Original languageEnglish
Pages (from-to)998-1008
Number of pages11
JournalJournal of Neurochemistry
Issue number4
StatePublished - Feb 2009


  • 3,4-dihydroxyphenyl-L-alanine
  • 3,4-dihydroxyphenyl-L-alanine induced dyskinesia
  • 5-hydroxytryptamine or serotonin
  • Chronoamperometry
  • Dopamine
  • Serotonin transporter

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience


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