Clathrin light chain B: Gene structure and neuron-specific splicing

Stefan Stamm, Diana Casper, Jonathan Dinsmore, Charles A. Kaufmann, Jürgen Brosius, David M. Helfman

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


The clathrin light chains are components of clathrin coated vesicles, structural constituents involved in endocytosls and membrane recycling. The clathrin light chain B (LCB) gene encodes two isoforms, termed LCB2 and LCB3, via an alternative RNA splicing mechanism. We have determined the structure of the rat clathrin light chain B gene. The gene consists of six exons that extend over 11.9 kb. The first four exons and the last exon are common to the LCB2 and LCB3 isoforms. The fifth exon, termed EN, is included in the mRNA in brain, giving rise to the brain specific form LCB2 but is excluded in other tissues, generating the LCB3 isoform. Primary rat neuronal cell cultures express predominantly the brain specific LCB2 isoform, whereas primary rat cultures of glia express only the LCB3 isoform, suggesting that expression of the brain-specific LCB2 form is limited to neurons. Further evidence for neuronal localization of the LCB2 form is provided using a teratocarcinoma cell line, P19, which can be induced by retinoic acid to express a neuronal phenotype, concomitant with the induction of the LCB2 form. In order to determine the sequences involved in alternative splice site selection, we constructed a minigene containing the alternative spliced exon EN and its flanking intron and exon sequences. This minigene reflects the splicing pattern of the endogenous gene upon transfection in HeLa cell and primary neuronal cell cultures, indicating that this region of the LCB gene contains all the necessary information for neuron-specific splicing.

Original languageEnglish
Pages (from-to)5097-5103
Number of pages7
JournalNucleic Acids Research
Issue number19
StatePublished - Oct 11 1992

Bibliographical note

Funding Information:
We thank R.Hynes for the XEMBL3B rat liver library. JD wants to thank M.McBurney and M.Rudnicki for providing P19 embryonal carcinoma cells. We thank H.Nawa for primary cortex, SCG and glial cultures. pTKB2 and pTKB3 were a generous gift from T.Kirchhausen. J.D. was supported by a postdoctoral fellowship from the American Cancer Society and by a National Cancer Institute grant to F.Solomon (MIT). C.A.K. is supported by a Physician Scientist Award NIMH Kll-MH 00682. S.S. is supported by a fellowship of the Gottlieb Daimler-and Karl Benz-Stiftung # 2.88.9. J.B. is supported by a grant from NIMH MH38819. D.M.H. is supported by a National Institutes of Health Grant R01-GM43040 and is an Established Investigator of the American Heart Association.

ASJC Scopus subject areas

  • Genetics


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