Cloning and analysis of the gene for the human puromycin-sensitive aminopeptidase

Michael W. Thompson; Andreas Tobler; Adriano Fontana; Louis B. Hersh

doi:10.1006/bbrc.1999.0604

Cloning and analysis of the gene for the human puromycin-sensitive aminopeptidase

Michael W. Thompson, Andreas Tobler, Adriano Fontana, Louis B. Hersh

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

The gene encoding the human puromycin-sensitive aminopeptidase (PSA) has been cloned and characterized. The human PSA gene is composed of 23 exons and 22 introns and spans approximately 40 kb of chromosome 17 at the interval 17q12-21. An analysis of the 5' end of the human PSA transcript reveals that the translational start site corresponds to nt 210 of the human PSA cDNA, as suggested by RT-PCR, 5' RACE, and computer analysis of expressed sequence tags. A comparison of the exon/exon boundaries of the human PSA gene with those of the human aminopeptidase N (APN) gene shows little conservation, suggesting that the two genes, which are closely related in protein sequence, diverged early during evolution.

Original language	English
Pages (from-to)	234-240
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	258
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1999.0604
State	Published - May 10 1999

Bibliographical note

Funding Information:
This work was supported in part by a grant from the NIH, NIDA02243. Mr. Thompson is supported by NIDA fellowship DA05764-03. We thank Drs. C. Kaetzel and J. N. Davidson for helpful discussions regarding the manuscript.

Keywords

Exon
Genomic cloning
Gluzincin
Intron
Metalloprotease

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1006/bbrc.1999.0604

Cite this

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title = "Cloning and analysis of the gene for the human puromycin-sensitive aminopeptidase",

abstract = "The gene encoding the human puromycin-sensitive aminopeptidase (PSA) has been cloned and characterized. The human PSA gene is composed of 23 exons and 22 introns and spans approximately 40 kb of chromosome 17 at the interval 17q12-21. An analysis of the 5' end of the human PSA transcript reveals that the translational start site corresponds to nt 210 of the human PSA cDNA, as suggested by RT-PCR, 5' RACE, and computer analysis of expressed sequence tags. A comparison of the exon/exon boundaries of the human PSA gene with those of the human aminopeptidase N (APN) gene shows little conservation, suggesting that the two genes, which are closely related in protein sequence, diverged early during evolution.",

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author = "Thompson, {Michael W.} and Andreas Tobler and Adriano Fontana and Hersh, {Louis B.}",

note = "Funding Information: This work was supported in part by a grant from the NIH, NIDA02243. Mr. Thompson is supported by NIDA fellowship DA05764-03. We thank Drs. C. Kaetzel and J. N. Davidson for helpful discussions regarding the manuscript.",

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AU - Tobler, Andreas

AU - Fontana, Adriano

AU - Hersh, Louis B.

N1 - Funding Information: This work was supported in part by a grant from the NIH, NIDA02243. Mr. Thompson is supported by NIDA fellowship DA05764-03. We thank Drs. C. Kaetzel and J. N. Davidson for helpful discussions regarding the manuscript.

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AB - The gene encoding the human puromycin-sensitive aminopeptidase (PSA) has been cloned and characterized. The human PSA gene is composed of 23 exons and 22 introns and spans approximately 40 kb of chromosome 17 at the interval 17q12-21. An analysis of the 5' end of the human PSA transcript reveals that the translational start site corresponds to nt 210 of the human PSA cDNA, as suggested by RT-PCR, 5' RACE, and computer analysis of expressed sequence tags. A comparison of the exon/exon boundaries of the human PSA gene with those of the human aminopeptidase N (APN) gene shows little conservation, suggesting that the two genes, which are closely related in protein sequence, diverged early during evolution.

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KW - Genomic cloning

KW - Gluzincin

KW - Intron

KW - Metalloprotease

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Cloning and analysis of the gene for the human puromycin-sensitive aminopeptidase

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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