Cloning and characterization of a novel human gene encoding a zinc finger protein with 25 fingers

Xiang An Li, Koichi Kokame, Kousaku Okubo, Kentaro Shimokado, Yoshitane Tsukamoto, Toshiyuki Miyata, Hisao Kato, Chikao Yutani

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

This study reports cloning and characterization of a human cDNA encoding a novel human zinc finger protein, ZFD25. ZFD25 cDNA is 6118 bp long and has an open reading frame of 2352 bp that encodes a 783 amino acid protein with 25 C2H2-type zinc fingers. The ZFD25 cDNA also contains a region with high sequence similarity to the Kruppel-associated box A and B domain in the 5'-untranslated region, suggesting that ZFD25 belongs to the Kruppel-associated box zinc finger protein family. The ZFD25 gene was localized to chromosome 7q11.2. Northern blot analysis showed that ZFD25 was expressed in a wide range of human organs. In cultured endothelial cells, the mRNA level was decreased upon serum starvation. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)405-412
Number of pages8
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1489
Issue number2-3
DOIs
StatePublished - Dec 23 1999

Bibliographical note

Funding Information:
The authors thank Dr. S. Kawamoto for her help in determining chromosomal localization, and Ms. H. Sugita and C. Yasuda for their excellent technical assistance. This work was supported by a grant from the Organization for Pharmaceutical Safety and Research of Japan and a grant from the Science and Technology Agency. The nucleotide sequence for ZFD25 can be found in the GenBank/EMBL/DDBJ database (accession no. AB027251).

Keywords

  • Human
  • KRAB
  • Zinc finger protein
  • cDNA

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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