Cloning and characterization of a novel human gene encoding a zinc finger protein with 25 fingers

Xiang An Li; Koichi Kokame; Kousaku Okubo; Kentaro Shimokado; Yoshitane Tsukamoto; Toshiyuki Miyata; Hisao Kato; Chikao Yutani

doi:10.1016/S0167-4781(99)00206-7

Cloning and characterization of a novel human gene encoding a zinc finger protein with 25 fingers

Xiang An Li, Koichi Kokame, Kousaku Okubo, Kentaro Shimokado, Yoshitane Tsukamoto, Toshiyuki Miyata, Hisao Kato, Chikao Yutani

Physiology

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

This study reports cloning and characterization of a human cDNA encoding a novel human zinc finger protein, ZFD25. ZFD25 cDNA is 6118 bp long and has an open reading frame of 2352 bp that encodes a 783 amino acid protein with 25 C2H2-type zinc fingers. The ZFD25 cDNA also contains a region with high sequence similarity to the Kruppel-associated box A and B domain in the 5'-untranslated region, suggesting that ZFD25 belongs to the Kruppel-associated box zinc finger protein family. The ZFD25 gene was localized to chromosome 7q11.2. Northern blot analysis showed that ZFD25 was expressed in a wide range of human organs. In cultured endothelial cells, the mRNA level was decreased upon serum starvation. Copyright (C) 1999 Elsevier Science B.V.

Original language	English
Pages (from-to)	405-412
Number of pages	8
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1489
Issue number	2-3
DOIs	https://doi.org/10.1016/S0167-4781(99)00206-7
State	Published - Dec 23 1999

Bibliographical note

Funding Information:
The authors thank Dr. S. Kawamoto for her help in determining chromosomal localization, and Ms. H. Sugita and C. Yasuda for their excellent technical assistance. This work was supported by a grant from the Organization for Pharmaceutical Safety and Research of Japan and a grant from the Science and Technology Agency. The nucleotide sequence for ZFD25 can be found in the GenBank/EMBL/DDBJ database (accession no. AB027251).

Keywords

Human
KRAB
Zinc finger protein
cDNA

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/S0167-4781(99)00206-7

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title = "Cloning and characterization of a novel human gene encoding a zinc finger protein with 25 fingers",

abstract = "This study reports cloning and characterization of a human cDNA encoding a novel human zinc finger protein, ZFD25. ZFD25 cDNA is 6118 bp long and has an open reading frame of 2352 bp that encodes a 783 amino acid protein with 25 C2H2-type zinc fingers. The ZFD25 cDNA also contains a region with high sequence similarity to the Kruppel-associated box A and B domain in the 5'-untranslated region, suggesting that ZFD25 belongs to the Kruppel-associated box zinc finger protein family. The ZFD25 gene was localized to chromosome 7q11.2. Northern blot analysis showed that ZFD25 was expressed in a wide range of human organs. In cultured endothelial cells, the mRNA level was decreased upon serum starvation. Copyright (C) 1999 Elsevier Science B.V.",

keywords = "Human, KRAB, Zinc finger protein, cDNA",

author = "Li, {Xiang An} and Koichi Kokame and Kousaku Okubo and Kentaro Shimokado and Yoshitane Tsukamoto and Toshiyuki Miyata and Hisao Kato and Chikao Yutani",

note = "Funding Information: The authors thank Dr. S. Kawamoto for her help in determining chromosomal localization, and Ms. H. Sugita and C. Yasuda for their excellent technical assistance. This work was supported by a grant from the Organization for Pharmaceutical Safety and Research of Japan and a grant from the Science and Technology Agency. The nucleotide sequence for ZFD25 can be found in the GenBank/EMBL/DDBJ database (accession no. AB027251).",

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AU - Li, Xiang An

AU - Kokame, Koichi

AU - Okubo, Kousaku

AU - Shimokado, Kentaro

AU - Tsukamoto, Yoshitane

AU - Miyata, Toshiyuki

AU - Kato, Hisao

AU - Yutani, Chikao

N1 - Funding Information: The authors thank Dr. S. Kawamoto for her help in determining chromosomal localization, and Ms. H. Sugita and C. Yasuda for their excellent technical assistance. This work was supported by a grant from the Organization for Pharmaceutical Safety and Research of Japan and a grant from the Science and Technology Agency. The nucleotide sequence for ZFD25 can be found in the GenBank/EMBL/DDBJ database (accession no. AB027251).

PY - 1999/12/23

Y1 - 1999/12/23

N2 - This study reports cloning and characterization of a human cDNA encoding a novel human zinc finger protein, ZFD25. ZFD25 cDNA is 6118 bp long and has an open reading frame of 2352 bp that encodes a 783 amino acid protein with 25 C2H2-type zinc fingers. The ZFD25 cDNA also contains a region with high sequence similarity to the Kruppel-associated box A and B domain in the 5'-untranslated region, suggesting that ZFD25 belongs to the Kruppel-associated box zinc finger protein family. The ZFD25 gene was localized to chromosome 7q11.2. Northern blot analysis showed that ZFD25 was expressed in a wide range of human organs. In cultured endothelial cells, the mRNA level was decreased upon serum starvation. Copyright (C) 1999 Elsevier Science B.V.

AB - This study reports cloning and characterization of a human cDNA encoding a novel human zinc finger protein, ZFD25. ZFD25 cDNA is 6118 bp long and has an open reading frame of 2352 bp that encodes a 783 amino acid protein with 25 C2H2-type zinc fingers. The ZFD25 cDNA also contains a region with high sequence similarity to the Kruppel-associated box A and B domain in the 5'-untranslated region, suggesting that ZFD25 belongs to the Kruppel-associated box zinc finger protein family. The ZFD25 gene was localized to chromosome 7q11.2. Northern blot analysis showed that ZFD25 was expressed in a wide range of human organs. In cultured endothelial cells, the mRNA level was decreased upon serum starvation. Copyright (C) 1999 Elsevier Science B.V.

KW - Human

KW - KRAB

KW - Zinc finger protein

KW - cDNA

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Cloning and characterization of a novel human gene encoding a zinc finger protein with 25 fingers

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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